ArticleMolecular endocrinology (Baltimore, Md.)2015
Genetic analysis of zebrafish gonadotropin (FSH and LH) functions by TALEN-mediated gene disruption.
Article in Molecular endocrinology (Baltimore, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 247 citations in OpenAlex.
- Cholecystokinin coordinates gonadotropin-dependent and independent pathways to orchestrate zebrafish gonadal development.Nature communications · 2026Article
- Genetic and functional characterization of AMH Signaling in Zebrafish - Evidence for Roles of Amh-Bmpr2a-Bmpr1bb Pathway in Controlling Gonadal Homeostasis.PLoS genetics · 2026Article
- Emerging Perspectives on Gonadotropin Regulation in Vertebrates Revealed by the Discovery of FSH-RH in Teleosts.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Insights into the role of Fsh signaling in ovarian differentiation ofZoological research · 2025Article
- Vitellogenin receptor mediates heat adaptability of oocyte development in mud crabs and zebrafish.Nature communications · 2025Article
- Hormonal dynamics reveal a stimulatory role for secretoneurin in zebrafish ovulation.PNAS nexus · 2025Article
- Disrupting Amh and androgen signaling reveals their distinct roles in zebrafish gonadal differentiation and gametogenesis.Communications biology · 2025Article
- Cholecystokinin (CCK) Is a Mediator Between Nutritional Intake and Gonadal Development in Teleosts.Cells · 2025Article
- N-carbamylglutamate supplementation improves laying performance of layers by regulating hypothalamic-pituitary-ovarian axis.Frontiers in veterinary science · 2025Article
- The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion.eLife · 2024Article
- Combined Pituitary Hormone Deficiency inInternational journal of molecular sciences · 2024Article
- Loss of Function of Vasoactive-intestinal Peptide Alters Sex Ratio and Reduces Male Reproductive Fitness in Zebrafish.Endocrinology · 2024Article
- Review
- Day length regulates gonadotrope proliferation and reproduction via an intra-pituitary pathway in the model vertebrate Oryzias latipes.Communications biology · 2024Article
- Disruption of Thyroid Hormone Receptor Thrab Leads to Female Infertility in Zebrafish.Endocrinology · 2024Article
- Loss of Fshr Prevents Testicular Maturation in Atlantic Salmon (Salmo salar L.).Endocrinology · 2024Article
- Effects of flowing water stimulation on hormone regulation during the maturation process ofFrontiers in physiology · 2024Article
- Genetic evidence for differential functions of figla and nobox in zebrafish ovarian differentiation and folliculogenesis.Communications biology · 2023Article
- Alterations in Gene Expression and the Fatty Acid Profile Impact but Do Not Compromise the In Vitro Maturation of Zebrafish (Animals : an open access journal from MDPI · 2023Article
- Rescue of bmp15 deficiency in zebrafish by mutation of inha reveals mechanisms of BMP15 regulation of folliculogenesis.PLoS genetics · 2023Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vertebrate reproduction is controlled by two gonadotropins (FSH and LH) from the pituitary. Despite numerous studies on FSH and LH in fish species, their functions in reproduction still remain poorly defined. This is partly due to the lack of powerful genetic approaches for functional studies in adult fish. This situation is now changing with the emergence of genome-editing technologies, especially Transcription Activator-Like Effector Nuclease (TALEN) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). In this study, we deleted the hormone-specific β-genes of both FSH and LH in the zebrafish using TALEN. This was followed by a phenotype analysis for key reproductive events, including gonadal differentiation, puberty onset, gametogenesis, final maturation, and fertility. FSH-deficient zebrafish (fshb(-/-)) were surprisingly fertile in both sexes; however, the development of both the ovary and testis was significantly delayed. In contrast, LH-deficient zebrafish (lhb(-/-)) showed normal gonadal growth, but the females failed to spawn and were therefore infertile. Using previtellogenic follicles as the marker, we observed a significant delay of puberty onset in the fshb mutant but not the lhb mutant females. Interestingly, FSH seemed to play a role in maintaining the female status because we repeatedly observed sexual reversal in the fshb mutant. Neither the fshb nor lhb mutation alone seemed to affect gonadal differentiation; however, the double mutation of the two genes led to all males, although the development of the testis was significantly delayed. In summary, our data confirmed some well-known functions of FSH and LH in fish while also providing evidence for novel functions, which would be difficult to reveal using traditional biochemical and physiological approaches.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.