Evidence map›Paper›PMID 25395242›Full record

ArticleMolecular medicine reports2015

Adipose tissue-derived mesenchymal stem cells differentiated into hepatocyte-like cells in vivo and in vitro.

Libo Yin, Yuhua Zhu, Jiangang Yang, Yijiang Ni, Zhao Zhou, Yu Chen, Lixing Wen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 86 citations in OpenAlex.

  1. Article
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  11. Measurement of the Adipose Stem Cells Cell Sheets Transmittance.Bioengineering (Basel, Switzerland) · 2021
    Article
  12. Review
  13. Review
  14. Review
  15. Article
  16. Article
  17. Review
  18. Article
  19. Review
  20. Stem Cell-Based Therapies for Liver Diseases: An Overview and Update.Tissue engineering and regenerative medicine · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Libo YinDepartment of Traumatic Surgery, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.
Yuhua ZhuDepartment of Traumatic Surgery, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.
Jiangang YangDepartment of Traumatic Surgery, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.
Yijiang NiDepartment of Traumatic Surgery, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.
Zhao ZhouDepartment of Traumatic Surgery, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.
Yu ChenDepartment of Traumatic Surgery, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.
Lixing WenDepartment of Traumatic Surgery, Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.
Nanjing Medical University · CNChangzhou No.2 People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell‑based therapy is a potential alternative to liver transplantation. The goal of the present study was to examine the in vivo and in vitro hepatic differentiation potential of adipose tissue‑derived mesenchymal stem cells (AT‑MSCs) and to explore its therapeutic use. AT‑MSCs were isolated and cultured with hepatic differentiation medium. Bioactivity assays were used to study the properties of AT‑MSCs. The morphology of differentiated AT‑MSCs in serum‑free hepatic differentiation medium changed into polygonal epithelial cells, while the morphology of AT‑MSCs in a similar medium containing 2% fetal bovine serum remained unchanged. The differentiated cells cultured without serum showed hepatocyte‑like cell morphology and hepatocyte‑specific markers, including albumin (ALB) and α‑fetoprotein. The bioactivity assays revealed that hepatocyte‑like cells could take up low‑density lipoprotein (LDL) and store glycogen. Furthermore, trichostatin A (TSA) enhanced ALB production and LDL uptake by the hepatocyte‑like cells, analogous to the functions of human liver cells. ALB was detected in the livers of the CCl4‑injured mice one month post‑transplantation. This suggested that transplantation of the human AT‑MSCs could relieve the impairment of acute CCl4‑injured livers in nude mice. This therefore implied that adipose tissue was a source of multipotent stem cells which had the potential to differentiate into mature, transplantable hepatocyte‑like cells in vivo and in vitro. In addition, the present study determined that TSA was essential to promoting differentiation of human MSC towards functional hepatocyte‑like cells. The relief of liver injury following treatment with AT‑MSCs suggested their potential as a novel therapeutic method for liver disorders or injury.

Indexed as

Cell DifferentiationMesenchymal Stem Cell TransplantationAdipose TissueAnimalsBiomarkersCell CycleChemical and Drug Induced Liver InjuryHepatocytesHumansLiver Function TestsMesenchymal Stem CellsMiceModels, AnimalPhenotypeBiomarkers

Identifiers

PMID25395242
PMCPMC4270341
OpenAlexW2171820242

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.