Evidence map›Paper›PMID 25394742›Full record

ArticleInternational journal of legal medicine2015

Isolating cells from female/male blood mixtures using florescence in situ hybridization combined with low volume PCR and its application in forensic science.

Lei Feng, Cai-Xia Li, Jun-Ping Han, Cheng Xu, Lan Hu

Abstract read
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In one paragraph

Article in International journal of legal medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lei FengKey Laboratory of Forensic Genetics, Ministry of Public Security, Institute of Forensic Science, Beijing, 100038, China.
Cai-Xia LiKey Laboratory of Forensic Genetics, Ministry of Public Security, Institute of Forensic Science, Beijing, 100038, China. 13810666929@139.com.
Jun-Ping HanChinese Peoples Public Security University, Beijing, 100038, China.
Cheng XuChinese Peoples Public Security University, Beijing, 100038, China.
Lan HuKey Laboratory of Forensic Genetics, Ministry of Public Security, Institute of Forensic Science, Beijing, 100038, China. 13911162826@139.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To obtain single-source short tandem repeat (STR) profiles in trace female/male blood mixture samples, we combined florescence in situ hybridization (FISH), laser microdissection, and low volume PCR (LV-PCR) to isolate male/female cells and improve sensitivity. The results showed that isolation of as few as 10 leukocytes was sufficient to yield full STR profiles in fresh female or male blood samples for 32 independent tests with a low additional alleles rate (3.91%) and drop-out alleles rate (5.01%). Moreover, this procedure was tested in two fresh blood mixture series at three ratios (1:5, 1:10, and 1:20), two mock female/male blood mixture casework samples, and one practical casework sample. Male and female STR profiles were successfully detected in all of these samples, showing that this procedure could be used in forensic casework in the future.

Indexed as

Microsatellite RepeatsBlood Cell CountChromosomes, Human, XChromosomes, Human, YDNA FingerprintingFemaleHumansIn Situ Hybridization, FluorescenceLaser Capture MicrodissectionLeukocytesMalePolymerase Chain ReactionDNA typingFemale/male blood mixtureFlorescence in situ hybridizationForensic scienceLow volume PCRSingle-cell capture

Identifiers

PMID25394742

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.