ArticleParasites & vectors2014
Identification of six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 99 citations in OpenAlex.
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- Emergence of autochthonousFrontiers in veterinary science · 2025Article
- Assessment of High-Resolution Melting Curve Analysis forPathogens (Basel, Switzerland) · 2024Article
- Prokaryotic and eukaryotic skin microbiota modifications triggered by Leishmania infection in localized Cutaneous Leishmaniasis.PLoS neglected tropical diseases · 2024Article
- Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis ofCurrent research in parasitology & vector-borne diseases · 2024Article
- Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein.Parasites & vectors · 2023Article
- HRM Accuracy and Limitations as a Species Typing Tool forInternational journal of molecular sciences · 2023Article
- Detection ofAnimals : an open access journal from MDPI · 2023Article
- Evaluation of the Diagnostic Sensitivity of the VIASURETransboundary and emerging diseases · 2023Article
- Accurate Identification ofJournal of arthropod-borne diseases · 2022Article
- High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia.Parasites & vectors · 2022Article
- Two Leishmania species separation targeting the ITS-rDNA and Cyt b genes by developing and evaluating HRM- qPCR.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Development of an Amplicon-Based Next-Generation Sequencing Protocol to IdentifyMicrobiology spectrum · 2021Article
- Measuring spatial co-occurrences of species potentially involved in Leishmania transmission cycles through a predictive and fieldwork approach.Scientific reports · 2021Article
- Identification ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- High resolution melting assay in discrimination of the main etiologic agents of leishmaniasis in Iran.Iranian journal of microbiology · 2021Article
- Distribution, treatment outcome and genetic diversity of Leishmania species in military personnel from Colombia with cutaneous leishmaniasis.BMC infectious diseases · 2020Article
- Laboratory Diagnosis of Cutaneous and Visceral Leishmaniasis: Current and Future Methods.Microorganisms · 2020Review
- One Health Approach to Leishmaniases: Understanding the Disease Dynamics through Diagnostic Tools.Pathogens (Basel, Switzerland) · 2020Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeishmaniases are tropical zoonotic diseases, caused by parasites from the genus Leishmania. New World (NW) species are related to sylvatic cycles although urbanization processes have been reported in some South American Countries such as Colombia. This eco-epidemiological complexity imposes a challenge to the detection of circulating parasite species, not only related to human cases but also infecting vectors and reservoirs. Currently, no harmonized methods have been deployed to discriminate the NW Leishmania species.
findingsHerein, we conducted a systematic and mechanistic High-Resolution Melting (HRM) assay targeted to HSP70 and ITS1. Specific primers were designed that coupled with a HRM analyses permitted to discriminate six NW Leishmania species. In order to validate the herein described algorithm, we included 35 natural isolates obtained from human cases, insect vectors and mammals. Our genotyping assay allowed the correct assignment of the six NW Leishmania species (L. mexicana, L. infantum (chagasi), L. amazonensis, L. panamensis, L. guyanensis and L. braziliensis) based on reference strains. When the algorithm was applied to a set of well-characterized strains by means of PCR-RFLP, MLEE and monoclonal antibodies (MA) we observed a tailored concordance between the HRM and PCR-RFLP/MLEE/MA (KI = 1.0). Additionally, we tested the limit of detection for the HRM method showing that this is able to detect at least 10 equivalent-parasites per mL.
conclusionsThis is a rapid and reliable method to conduct molecular epidemiology and host-parasite association studies in endemic areas.
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