ArticleScientific reports2014
Rapid measurement of molecular transport and interaction inside living cells using single plane illumination.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- HIV-1 transmission: modelling and direct visualization in the third dimension.Microscopy (Oxford, England) · 2023Review
- SPIM-Flow: An Integrated Light Sheet and Microfluidics Platform for Hydrodynamic Studies ofBiology · 2023Article
- Breaking the Concentration Limit in Fluorescence Fluctuation Spectroscopy with Camera-Based Detection.International journal of molecular sciences · 2022Article
- miniSPIM-A Miniaturized Light-Sheet Microscope.ACS sensors · 2021Article
- Disruption of membrane cholesterol organization impairs the activity of PIEZO1 channel clusters.The Journal of general physiology · 2020Article
- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- Pair Correlation Analysis Maps the Dynamic Two-Dimensional Organization of Natural Killer Cell Receptors at the Synapse.ACS nano · 2019Article
- Selective plane illumination microscopy with a light sheet of uniform thickness formed by an electrically tunable lens.Microscopy research and technique · 2018Article
- Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions.Biomedical optics express · 2018Article
- sideSPIM - selective plane illumination based on a conventional inverted microscope.Biomedical optics express · 2017Article
- Measurement of Rapid Protein Diffusion in the Cytoplasm by Photo-Converted Intensity Profile Expansion.Cell reports · 2017Article
- 3D Protein Dynamics in the Cell Nucleus.Biophysical journal · 2017Article
- Diffusion Tensor Analysis by Two-Dimensional Pair Correlation of Fluorescence Fluctuations in Cells.Biophysical journal · 2016Article
- 4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM).Scientific reports · 2016Article
- Intracellular kinetics of the androgen receptor shown by multimodal Image Correlation Spectroscopy (mICS).Scientific reports · 2016Article
- Assessing the imaging performance of light sheet microscopies in highly scattering tissues.Biomedical optics express · 2016Article
- 3D fluorescence anisotropy imaging using selective plane illumination microscopy.Optics express · 2015Article
- Active focus stabilization for upright selective plane illumination microscopy.Optics express · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The ability to measure biomolecular dynamics within cells and tissues is very important to understand fundamental physiological processes including cell adhesion, signalling, movement, division or metabolism. Usually, such information is obtained using particle tracking methods or single point fluctuation spectroscopy. We show that image mean square displacement analysis, applied to single plane illumination microscopy data, is a faster and more efficient way of unravelling rapid, three-dimensional molecular transport and interaction within living cells. From a stack of camera images recorded in seconds, the type of dynamics such as free diffusion, flow or binding can be identified and quantified without being limited by current camera frame rates. Also, light exposure levels are very low and the image mean square displacement method does not require calibration of the microscope point spread function. To demonstrate the advantages of our approach, we quantified the dynamics of several different proteins in the cyto- and nucleoplasm of living cells. For example, from a single measurement, we were able to determine the diffusion coefficient of free clathrin molecules as well as the transport velocity of clathrin-coated vesicles involved in endocytosis. Used in conjunction with dual view detection, we further show how protein-protein interactions can be quantified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.