ArticleNature protocols2014
Differential centrifugation-based biochemical fractionation of the Drosophila adult CNS.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Protocol for preparation of a synaptosome-enriched fraction to profile presynaptic RNA content in adult Drosophila brains.STAR protocols · 2026Article
- Active zone plasticity couples sleep need to presynaptic hypophosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Myosin 15 participates in assembly and remodeling of the presynapse.The Journal of cell biology · 2025Article
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- Blobby is a synaptic active zone assembly protein required for memory in Drosophila.Nature communications · 2025Article
- Histamine synthesis and transport are coupled in axon terminals via a dual quality control system.The EMBO journal · 2024Article
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- Enrichment of Membrane Proteins for Downstream Analysis Using Styrene Maleic Acid Lipid Particles (SMALPs) Extraction.Bio-protocol · 2023Article
- Interactive nanocluster compaction of the ELKS scaffold and Cacophony CaScience advances · 2023Article
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- A Non-Nuclear NF-κB Modulates Alcohol Sensitivity But Not Immunity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Cleavage of human tau at Asp421 inhibits hyperphosphorylated tau induced pathology in a Drosophila model.Scientific reports · 2020Article
- RIM-binding protein couples synaptic vesicle recruitment to release sites.The Journal of cell biology · 2020Article
- Translation of CircRNAs.Molecular cell · 2017Article
- Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function.Nature communications · 2015Article
- Putative synaptic genes defined from a Drosophila whole body developmental transcriptome by a machine learning approach.BMC genomics · 2015Article
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Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila is widely used as a genetic model in questions of development, cellular function and disease. Genetic screens in flies have proven to be incredibly powerful in identifying crucial components for synapse formation and function, particularly in the case of the presynaptic release machinery. Although modern biochemical methods can identify individual proteins and lipids (and their binding partners), they have typically been excluded from use in Drosophila for technical reasons. To bridge this essential gap between genetics and biochemistry, we developed a fractionation method to isolate various parts of the synaptic machinery from Drosophila, thus allowing it to be studied in unprecedented biochemical detail. This is only possible because our protocol has unique advantages in terms of enriching and preserving endogenous protein complexes. The procedure involves decapitation of adult flies, homogenization and differential centrifugation of fly heads, which allow subsequent purification of presynaptic (and to a limited degree postsynaptic) components. It is designed to require only a rudimentary knowledge of biochemical fractionation, and it takes ∼3.5 h. The yield is typically 4 mg of synaptic membrane protein per gram of Drosophila heads.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.