ArticlePLoS computational biology2014
Inferring viral dynamics in chronically HCV infected patients from the spatial distribution of infected hepatocytes.
Article in PLoS computational biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- Direct-Acting Antivirals Quickly Eradicate Hepatitis C Virus From the Liver in People With Human Immunodeficiency Virus but Do Not Fully Reverse Immune Activation.The Journal of infectious diseases · 2025Trial
- Second-Phase Hepatitis C Plasma Viral Kinetics Directly Reflects Reduced Intrahepatic Burden of Hepatitis C Virus.The Journal of infectious diseases · 2023Trial
- Intrahepatic Viral Kinetics During Direct-Acting Antivirals for Hepatitis C in Human Immunodeficiency Virus Coinfection: The AIDS Clinical Trials Group A5335S Substudy.The Journal of infectious diseases · 2020Trial
- Single-Cell Hepatitis B Sequencing Reveals Distinct Viral Infection Events Consistent With Superinfection.The Journal of infectious diseases · 2026Article
- Transcriptomic and Zonal Signatures of Mitochondrial Peroxisomal Dysfunction in HCV Associates with Circulating Mitochondrial DNA Biomarkers.Current issues in molecular biology · 2026Article
- Spatially structured models of viral dynamics: a scoping review.Microbiology and molecular biology reviews : MMBR · 2025Article
- Article
- HCV Spread Kinetics Reveal Varying Contributions of Transmission Modes to Infection Dynamics.Viruses · 2021Article
- Autocrine and paracrine interferon signalling as 'ring vaccination' and 'contact tracing' strategies to suppress virus infection in a host.Proceedings. Biological sciences · 2021Article
- HIV influences clustering and intracellular replication of hepatitis C virus.Journal of viral hepatitis · 2021Article
- Single hepatocytes show persistence and transcriptional inactivity of hepatitis B.JCI insight · 2020Article
- Modeling the efficiency of filovirus entry into cells in vitro: Effects of SNP mutations in the receptor molecule.PLoS computational biology · 2020Article
- Persistent Innate Immune Stimulation Results in IRF3-Mediated but Caspase-Independent Cytostasis.Viruses · 2020Article
- Review
- Single Hepatocyte Hepatitis B Virus Transcriptional Landscape in HIV Coinfection.The Journal of infectious diseases · 2020Article
- Building a mechanistic mathematical model of hepatitis C virus entry.PLoS computational biology · 2019Article
- Principles of Effective and Robust Innate Immune Response to Viral Infections: A Multiplex Network Analysis.Frontiers in immunology · 2019Article
- Review
- Acute hepatitis B virus infection in humanized chimeric mice has multiphasic viral kinetics.Hepatology (Baltimore, Md.) · 2018Article
- Superinfection and cure of infected cells as mechanisms for hepatitis C virus adaptation and persistence.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Chronic liver infection by hepatitis C virus (HCV) is a major public health concern. Despite partly successful treatment options, several aspects of intrahepatic HCV infection dynamics are still poorly understood, including the preferred mode of viral propagation, as well as the proportion of infected hepatocytes. Answers to these questions have important implications for the development of therapeutic interventions. In this study, we present methods to analyze the spatial distribution of infected hepatocytes obtained by single cell laser capture microdissection from liver biopsy samples of patients chronically infected with HCV. By characterizing the internal structure of clusters of infected cells, we are able to evaluate hypotheses about intrahepatic infection dynamics. We found that individual clusters on biopsy samples range in size from 4-50 infected cells. In addition, the HCV RNA content in a cluster declines from the cell that presumably founded the cluster to cells at the maximal cluster extension. These observations support the idea that HCV infection in the liver is seeded randomly (e.g. from the blood) and then spreads locally. Assuming that the amount of intracellular HCV RNA is a proxy for how long a cell has been infected, we estimate based on models of intracellular HCV RNA replication and accumulation that cells in clusters have been infected on average for less than a week. Further, we do not find a relationship between the cluster size and the estimated cluster expansion time. Our method represents a novel approach to make inferences about infection dynamics in solid tissues from static spatial data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.