ArticlePloS one2014
Distinct strains of Toxoplasma gondii feature divergent transcriptomes regardless of developmental stage.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Advances towards the completeFaculty reviews · 2023Review
- Restriction Checkpoint Controls Bradyzoite Development in Toxoplasma gondii.Microbiology spectrum · 2022Article
- Genome-wide localization of histone variants in Toxoplasma gondii implicates variant exchange in stage-specific gene expression.BMC genomics · 2022Article
- Transcriptomics analysis of Toxoplasma gondii-infected mouse macrophages reveals coding and noncoding signatures in the presence and absence of MyD88.BMC genomics · 2021Article
- Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection byPathogens (Basel, Switzerland) · 2020Article
- Cell type- and species-specific host responses to Toxoplasma gondii and its near relatives.International journal for parasitology · 2020Review
- Label-Free Quantitative Acetylome Analysis RevealsMicroorganisms · 2019Article
- ApiAP2 Transcription Factors in Apicomplexan Parasites.Pathogens (Basel, Switzerland) · 2019Review
- Gene target discovery with network analysis in Toxoplasma gondii.Scientific reports · 2019Article
- A latent ability to persist: differentiation in Toxoplasma gondii.Cellular and molecular life sciences : CMLS · 2018Review
- Dissection of theeLife · 2018Article
- The Expressed MicroRNA-mRNA Interactions ofFrontiers in microbiology · 2017Article
- Sexual development in Plasmodium parasites: knowing when it's time to commit.Nature reviews. Microbiology · 2015Review
- Integration of RNA-seq and proteomics data with genomics for improved genome annotation in Apicomplexan parasites.Proteomics · 2015Article
- toxoMine: an integrated omics data warehouse for Toxoplasma gondii systems biology research.Database : the journal of biological databases and curation · 2015Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Using high through-put RNA sequencing, we assayed the transcriptomes of three different strains of Toxoplasma gondii representing three common genotypes under both in vitro tachyzoite and in vitro bradyzoite-inducing alkaline stress culture conditions. Strikingly, the differences in transcriptional profiles between the strains, RH, PLK, and CTG, is much greater than differences between tachyzoites and alkaline stressed in vitro bradyzoites. With an FDR of 10%, we identified 241 genes differentially expressed between CTG tachyzoites and in vitro bradyzoites, including 5 putative AP2 transcription factors. We also observed a close association between cell cycle regulated genes and differentiation. By Gene Set Enrichment Analysis (GSEA), there are a number of KEGG pathways associated with the in vitro bradyzoite transcriptomes of PLK and CTG, including pyrimidine metabolism and DNA replication. These functions are likely associated with cell-cycle arrest. When comparing mRNA levels between strains, we identified 1,526 genes that were differentially expressed regardless of culture-condition as well as 846 differentially expressed only in bradyzoites and 542 differentially expressed only in tachyzoites between at least two strains. Using GSEA, we identified that ribosomal proteins were expressed at significantly higher levels in the CTG strain than in either the RH or PLK strains. This association holds true regardless of life cycle stage.
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Registered trials
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