ArticlePloS one2014
Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Culture and transfection: Two major bottlenecks in understandingFrontiers in microbiology · 2023Review
- The challenges ofFrontiers in immunology · 2022Article
- Advancing Key Gaps in the Knowledge ofFrontiers in cellular and infection microbiology · 2022Review
- Generation of red blood cells from stem cells: Achievements, opportunities and perspectives for malaria research.Frontiers in cellular and infection microbiology · 2022Review
- A Way Forward for Culturing Plasmodium vivax.Trends in parasitology · 2020Review
- Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes.Scientific reports · 2019Article
- malERA: An updated research agenda for basic science and enabling technologies in malaria elimination and eradication.PLoS medicine · 2017Review
- The Biology ofCold Spring Harbor perspectives in medicine · 2017Review
- Malaria modeling: In vitro stem cells vs in vivo models.World journal of stem cells · 2016Review
- Improvement of culture conditions for long-term in vitro culture of Plasmodium vivax.Malaria journal · 2015Article
- Killing the hypnozoite--drug discovery approaches to prevent relapse in Plasmodium vivax.Pathogens and global health · 2015Review
- Residues 39-56 of Stem Cell Factor Protein Sequence Are Capable of Stimulating the Expansion of Cord Blood CD34+ Cells.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.
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Registered trials
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