ArticlePloS one2014
Comparison of longitudinal in vivo measurements of retinal nerve fiber layer thickness and retinal ganglion cell density after optic nerve transection in rat.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Optic Nerve Crush Does Not Induce Retinal Ganglion Cell Loss in the Contralateral Eye.Investigative ophthalmology & visual science · 2025Article
- Development of Microcystoid Macular Degeneration in the Retina of Nonhuman Primates: Time-Course and Associated Pathologies.Current eye research · 2025Article
- Retinal Input to Macaque Superior Colliculus Derives from Branching Axons Projecting to the Lateral Geniculate Nucleus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- BDNF and cAMP are neuroprotective in a porcine model of traumatic optic neuropathy.JCI insight · 2024Article
- Longitudinal Observation of Retinal Response to Optic Nerve Transection in Rats Using Visible Light Optical Coherence Tomography.Investigative ophthalmology & visual science · 2023Article
- Progression of optic atrophy in traumatic optic neuropathy: retrograde neuronal degeneration in humans.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2022Article
- Mechanisms implicated in the contralateral effect in the central nervous system after unilateral injury: focus on the visual system.Neural regeneration research · 2021Review
- Establishing the ground squirrel as a superb model for retinal ganglion cell disorders and optic neuropathies.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Longitudinal outcomes of circumlimbal suture model-induced chronic ocular hypertension in Sprague-Dawley albino rats.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2020Article
- The effect of retrobulbar anesthesia for cataract surgery on the retinal nerve fiber layer thickness.International ophthalmology · 2020Article
- Activation of adenosine ACell death & disease · 2020Article
- Response of the Retinal Nerve Fiber Layer Reflectance and Thickness to Optic Nerve Crush.Investigative ophthalmology & visual science · 2018Article
- Article
- Ocular fundus pulsations within the posterior rat eye: Chorioscleral motion and response to elevated intraocular pressure.Scientific reports · 2017Article
- Assessing retinal ganglion cell damage.Eye (London, England) · 2017Review
- Shared and Differential Retinal Responses against Optic Nerve Injury and Ocular Hypertension.Frontiers in neuroscience · 2017Review
- Posterior rat eye during acute intraocular pressure elevation studied using polarization sensitive optical coherence tomography.Biomedical optics express · 2017Article
- Evaluating retinal ganglion cell loss and dysfunction.Experimental eye research · 2016Review
- In vivo imaging methods to assess glaucomatous optic neuropathy.Experimental eye research · 2015Review
- Retinal nerve fiber layer and ganglion cell complex thicknesses measured with spectral-domain optical coherence tomography in eyes with no light perception due to nonglaucomatous optic neuropathy.Japanese journal of ophthalmology · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
purposeTo determine the relationship between longitudinal in vivo measurements of retinal nerve fiber layer thickness (RNFLT) and retinal ganglion cell (RGC) density after unilateral optic nerve transection (ONT).
methodsNineteen adult Brown-Norway rats were studied; N = 10 ONT plus RGC label, N = 3 ONT plus vehicle only (sans label), N = 6 sham ONT plus RGC label. RNFLT was measured by spectral domain optical coherence tomography (SD-OCT) at baseline then weekly for 1 month. RGCs were labeled by retrograde transport of fluorescently conjugated cholera toxin B (CTB) from the superior colliculus 48 hours prior to ONT or sham surgery. RGC density measurements were obtained by confocal scanning laser ophthalmoscopy (CSLO) at baseline and weekly for 1 month. RGC density and reactivity of microglia (anti-Iba1) and astrocytes (anti-GFAP) were determined from post mortem fluorescence microscopy of whole-mount retinae.
resultsRNFLT decreased after ONT by 17% (p<0.05), 30% (p<0.0001) and 36% (p<0.0001) at weeks 2, 3 and 4. RGC density decreased after ONT by 18%, 69%, 85% and 92% at weeks 1, 2, 3 and 4 (p<0.0001 each). RGC density measured in vivo at week 4 and post mortem by microscopy were strongly correlated (R = 0.91, p<0.0001). In vivo measures of RNFLT and RGC density were strongly correlated (R = 0.81, p<0.0001). In ONT-CTB labeled fellow eyes, RNFLT increased by 18%, 52% and 36% at weeks 2, 3 and 4 (p<0.0001), but did not change in fellow ONT-eyes sans CTB. Microgliosis was evident in the RNFL of the ONT-CTB fellow eyes, exceeding that observed in other fellow eyes.
conclusionsIn vivo measurements of RNFLT and RGC density are strongly correlated and can be used to monitor longitudinal changes after optic nerve injury. The strong fellow eye effect observed in eyes contralateral to ONT, only in the presence of CTB label, consisted of a dramatic increase in RNFLT associated with retinal microgliosis.
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