Evidence map›Paper›PMID 25392912›Full record

ArticlePloS one2014

High phylogenetic diversity of glycosyl hydrolase family 10 and 11 xylanases in the sediment of Lake Dabusu in China.

Guozeng Wang, Xiaoyun Huang, Tzi Bun Ng, Juan Lin, Xiu Yun Ye

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Guozeng WangCollege of Biological Science and Technology, Fuzhou University, Fuzhou 350108, P.R. China; National Engineering Laboratory for High-efficiency Enzyme Expression, Fuzhou 350002, P. R. China.
Xiaoyun HuangCollege of Biological Science and Technology, Fuzhou University, Fuzhou 350108, P.R. China.
Tzi Bun NgSchool of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Juan LinCollege of Biological Science and Technology, Fuzhou University, Fuzhou 350108, P.R. China; National Engineering Laboratory for High-efficiency Enzyme Expression, Fuzhou 350002, P. R. China.
Xiu Yun YeCollege of Biological Science and Technology, Fuzhou University, Fuzhou 350108, P.R. China; National Engineering Laboratory for High-efficiency Enzyme Expression, Fuzhou 350002, P. R. China.
Fuzhou University · CNChinese University of Hong Kong · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Soda lakes are one of the most stable naturally occurring alkaline and saline environments, which harbor abundant microorganisms with diverse functions. In this study, culture-independent molecular methods were used to explore the genetic diversity of glycoside hydrolase (GH) family 10 and GH11 xylanases in Lake Dabusu, a soda lake with a pH value of 10.2 and salinity of 10.1%. A total of 671 xylanase gene fragments were obtained, representing 78 distinct GH10 and 28 GH11 gene fragments respectively, with most of them having low homology with known sequences. Phylogenetic analysis revealed that the GH10 xylanase sequences mainly belonged to Bacteroidetes, Proteobacteria, Actinobacteria, Firmicutes and Verrucomicrobia, while the GH11 sequences mainly consisted of Actinobacteria, Firmicutes and Fungi. A full-length GH10 xylanase gene (xynAS10-66) was directly cloned and expressed in Escherichia coli, and the recombinant enzymes showed high activity at alkaline pH. These results suggest that xylanase gene diversity within Lake Dabusu is high and that most of the identified genes might be novel, indicating great potential for applications in industry and agriculture.

Indexed as

PhylogenyActinobacteriaBacterial ProteinsBacteroidetesBiodiversityChinaEndo-1,4-beta XylanasesEnzyme AssaysEscherichia coliGene ExpressionGeologic SedimentsHydrogen-Ion ConcentrationIsoenzymesLakesMicrobial ConsortiaProteobacteriaBacterial ProteinsEndo-1,4-beta XylanasesIsoenzymesRecombinant Proteins

Identifiers

PMID25392912
PMCPMC4231106
OpenAlexW2016625480

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.