ArticlePlant & cell physiology2015
Multigene knockout utilizing off-target mutations of the CRISPR/Cas9 system in rice.
Article in Plant & cell physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
90 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Which Factors Affect the Occurrence of Off-Target Effects Caused by the Use of CRISPR/Cas: A Systematic Review in Plants.Frontiers in plant science · 2020Pooled it
- Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement.Plant cell reports · 2026Review
- OsFPN1 functions as an essential metal transporter for Fe homeostasis and Co/Ni tolerance in rice.Plant molecular biology · 2026Article
- OsAux1 is required for nutritropism in rice roots.The Plant journal : for cell and molecular biology · 2026Article
- Florigen and cytokinin signaling antagonistically regulate FLOWERING LOCUS T-LIKE1 to drive a florigen relay that facilitates inflorescence development in rice.Science advances · 2025Article
- Kingdom-wide CRISPR guide design with ALLEGRO.Nucleic acids research · 2025Article
- Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level.Nature communications · 2025Article
- A CRISPR-HITI strategy approach to improve CHO cell viability by modifying the 3'UTR of Caspase 8 Associated Protein 2.Molecular biology research communications · 2025Article
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- CRISPR-M: Predicting sgRNA off-target effect using a multi-view deep learning network.PLoS computational biology · 2024Article
- Genome editing in rice using CRISPR/Cas12i3.Plant biotechnology journal · 2024Article
- GWAS for the identification of introgressed candidate genes ofFrontiers in plant science · 2024Article
- Novel Salinity-Tolerant Third-Generation Hybrid Rice Developed via CRISPR/Cas9-Mediated Gene Editing.International journal of molecular sciences · 2023Article
- Genetic basis controlling rice plant architecture and its modification for breeding.Breeding science · 2023Article
- Application of CRISPR/Cas9 Technology in Cancer Treatment: A Future Direction.Current oncology (Toronto, Ont.) · 2023Review
- General guidelines for CRISPR/Cas-based genome editing in plants.Molecular biology reports · 2022Review
- Genome-wide association study identifies a gene responsible for temperature-dependent rice germination.Nature communications · 2022Article
- Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA.Plant physiology · 2022Article
- CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement.International journal of molecular sciences · 2022Review
- Boron uptake in rice is regulated post-translationally via a clathrin-independent pathway.Plant physiology · 2022Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has been demonstrated to be a robust genome engineering tool in a variety of organisms including plants. However, it has been shown that the CRISPR/Cas9 system cleaves genomic DNA sequences containing mismatches to the guide RNA strand. We expected that this low specificity could be exploited to induce multihomeologous and multiparalogous gene knockouts. In the case of polyploid plants, simultaneous modification of multiple homeologous genes, i.e. genes with similar but not identical DNA sequences, is often needed to obtain a desired phenotype. Even in diploid plants, disruption of multiparalogous genes, which have functional redundancy, is often needed. To validate the applicability of the CRISPR/Cas9 system to target mutagenesis of paralogous genes in rice, we designed a single-guide RNA (sgRNA) that recognized 20 bp sequences of cyclin-dependent kinase B2 (CDKB2) as an on-target locus. These 20 bp possess similarity to other rice CDK genes (CDKA1, CDKA2 and CDKB1) with different numbers of mismatches. We analyzed mutations in these four CDK genes in plants regenerated from Cas9/sgRNA-transformed calli and revealed that single, double and triple mutants of CDKA2, CDKB1 and CDKB2 can be created by a single sgRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.