ArticleMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada2015
X-ray microscopy as an approach to increasing accuracy and efficiency of serial block-face imaging for correlated light and electron microscopy of biological specimens.
Article in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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Who cites it
38 citing papers in PubMed.
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- Inhibitory neurons marked by the connectivity molecule Kirrel3 regulate memory precision.bioRxiv : the preprint server for biology · 2025Article
- 3D X-ray microscope acts as an accurate and effective equipment of pathological diagnosis in craniofacial imaging.Scientific reports · 2024Article
- New rabies viral resources for multi-scale neural circuit mapping.Molecular psychiatry · 2024Article
- Morphomics via next-generation electron microscopy.Journal of molecular cell biology · 2024Review
- Plant microphenotype: from innovative imaging to computational analysis.Plant biotechnology journal · 2024Review
- Crosshair, semi-automated targeting for electron microscopy with a motorised ultramicrotome.eLife · 2022Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy.Nature communications · 2022Article
- X-ray microscopy enables multiscale high-resolution 3D imaging of plant cells, tissues, and organs.Plant physiology · 2022Article
- Sample Preparation and Warping Accuracy for Correlative Multimodal Imaging in the Mouse Olfactory Bulb Using 2-Photon, Synchrotron X-Ray and Volume Electron Microscopy.Frontiers in cell and developmental biology · 2022Article
- Field-Emission Scanning Electron Microscope as a Tool for Large-Area and Large-Volume Ultrastructural Studies.Animals : an open access journal from MDPI · 2021Review
- High-Resolution Multi-Isotope Imaging Mass Spectrometry (MIMS) Imaging Applications in Stem Cell Biology.Current protocols · 2021Article
- Correlative light and volume electron microscopy (vCLEM): How community participation can advance developing technologies.Journal of microscopy · 2021Article
- Taking a microscale look at symbiotic interactions-and why it matters.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits.eLife · 2021Article
- Assaying three-dimensional cellular architecture using X-ray tomographic and correlated imaging approaches.The Journal of biological chemistry · 2020Review
- Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution.iScience · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The recently developed three-dimensional electron microscopic (EM) method of serial block-face scanning electron microscopy (SBEM) has rapidly established itself as a powerful imaging approach. Volume EM imaging with this scanning electron microscopy (SEM) method requires intense staining of biological specimens with heavy metals to allow sufficient back-scatter electron signal and also to render specimens sufficiently conductive to control charging artifacts. These more extreme heavy metal staining protocols render specimens light opaque and make it much more difficult to track and identify regions of interest (ROIs) for the SBEM imaging process than for a typical thin section transmission electron microscopy correlative light and electron microscopy study. We present a strategy employing X-ray microscopy (XRM) both for tracking ROIs and for increasing the efficiency of the workflow used for typical projects undertaken with SBEM. XRM was found to reveal an impressive level of detail in tissue heavily stained for SBEM imaging, allowing for the identification of tissue landmarks that can be subsequently used to guide data collection in the SEM. Furthermore, specific labeling of individual cells using diaminobenzidine is detectable in XRM volumes. We demonstrate that tungsten carbide particles or upconverting nanophosphor particles can be used as fiducial markers to further increase the precision and efficiency of SBEM imaging.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.