ArticleNature communications2014
Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
98 citing papers in PubMed.
- Artificial Intelligence-Empowered Single-Cell Phenotyping for Rapid, Automated Pathogen Diagnostics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A Droplet-Based Microfluidic Platform for Rapid Optical Detection of Bacteria: Proof-of-Concept for Radiopharmaceutical Sterility Testing.Micromachines · 2026Article
- Rapid detection and capture of clinical Escherichia coli strains mediated by OmpA-targeting nanobodies.Communications biology · 2025Article
- Droplet microfluidics-based detection of rare antibiotic-resistant subpopulations inScience advances · 2025Article
- Targeted recognition, fluorescent tracking and augmented killing of multi-bacterial infectionsChemical science · 2025Article
- Synergistic detection of E. coli using ultrathin film of functionalized graphene with impedance spectroscopy and machine learning.Scientific reports · 2025Article
- Biorecognition-Based Nanodiagnostics: Maltotriose-Functionalized Magnetic Nanoparticles for Targeted Magnetic Resonance Imaging of Bacterial Infections.Bioengineering (Basel, Switzerland) · 2025Article
- Coupling of an Au@AgPt nanozyme array with an micrococcal nuclease-specific responsiveness strategy for colorimetric/SERS sensing ofJournal of pharmaceutical analysis · 2025Article
- The immunosuppressive mechanisms induced by sepsis and the corresponding treatment strategies.Frontiers in immunology · 2025Review
- Needle-Plug/Piston-Based Modular Mesoscopic Design Paradigm Coupled With Microfluidic Device for Point-of-Care Pooled Testing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Rapid diagnosis of bloodstream infections using a culture-free phenotypic platform.Communications medicine · 2024Article
- A DNAzymes-in-droplets assay forChemical science · 2024Article
- Biomolecular sensors for advanced physiological monitoring.Nature reviews bioengineering · 2023Review
- Absorption/Attenuation Spectral Description of ESKAPEE Bacteria: Application to Seeder-Free Culture Monitoring, Mammalian T-Cell and Bacteria Mixture Analysis and Contamination Description.Sensors (Basel, Switzerland) · 2023Article
- Analytical methods for assessing antimicrobial activity of nanomaterials in complex media: advances, challenges, and perspectives.Journal of nanobiotechnology · 2023Review
- Infection-specific PET imaging withFrontiers in microbiology · 2023Article
- Paper-based sensors for bacteria detection.Nature reviews bioengineering · 2023Review
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Engineering a SERS Sensing Nanoplatform with Self-Sterilization for Undifferentiated and Rapid Detection of Bacteria.Biosensors · 2023Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Blood stream infection or sepsis is a major health problem worldwide, with extremely high mortality, which is partly due to the inability to rapidly detect and identify bacteria in the early stages of infection. Here we present a new technology termed 'Integrated Comprehensive Droplet Digital Detection' (IC 3D) that can selectively detect bacteria directly from milliliters of diluted blood at single-cell sensitivity in a one-step, culture- and amplification-free process within 1.5-4 h. The IC 3D integrates real-time, DNAzyme-based sensors, droplet microencapsulation and a high-throughput 3D particle counter system. Using Escherichia coli as a target, we demonstrate that the IC 3D can provide absolute quantification of both stock and clinical isolates of E. coli in spiked blood within a broad range of extremely low concentration from 1 to 10,000 bacteria per ml with exceptional robustness and limit of detection in the single digit regime.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.