ArticleMethods in molecular biology (Clifton, N.J.)2015
Two-photon excitation microscopy and its applications in neuroscience.
Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed.
- Cortical circuit implementation of signed deviant detection with positive and negative prediction error neurons.bioRxiv : the preprint server for biology · 2026Article
- Advanced imaging strategies in cardiac organoids: bridging the gap between structural complexity and functional analysis.Cardiovascular diabetology · 2026Review
- Standardized measurements for monitoring and comparing multiphoton microscope systems.Nature protocols · 2025Review
- Arteries and veins in awake mice using two-photon microscopy.Journal of anatomy · 2025Article
- Photoactivated Protein Degrader for Optical Control of Synaptic Function.ACS chemical neuroscience · 2023Article
- A Photoactivated Protein Degrader for Optical Control of Synaptic Function.bioRxiv : the preprint server for biology · 2023Article
- Structural and functional imaging of brains.Science China. Chemistry · 2023Review
- Two-photon excitation fluorescence in ophthalmology: safety and improved imaging for functional diagnostics.Frontiers in medicine · 2023Review
- Dual GRIN lens two-photon endoscopy for high-speed volumetric and deep brain imaging.Biomedical optics express · 2021Article
- LED Zappelin': An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging.HardwareX · 2020Article
- Advances in adaptive optics-based two-photon fluorescence microscopy for brain imaging.Lasers in medical science · 2020Review
- EZcalcium: Open-Source Toolbox for Analysis of Calcium Imaging Data.Frontiers in neural circuits · 2020Article
- Recent trends in two-photon auto-fluorescence lifetime imaging (2P-FLIM) and its biomedical applications.Biomedical engineering letters · 2019Review
- Two-photon probes for in vivo multicolor microscopy of the structure and signals of brain cells.Brain structure & function · 2018Review
- Optogenetic approaches for dissecting neuromodulation and GPCR signaling in neural circuits.Current opinion in pharmacology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Two-photon excitation (2PE) overcomes many challenges in fluorescence microscopy. Compared to confocal microscopy, 2PE microscopy improves depth penetration, owing to the longer excitation wavelength required and to the ability to collect scattered emission photons as a useful signal. It also minimizes photodamage because lower energy photons are used and because fluorescence is confined to the geometrical focus of the laser spot. 2PE is therefore ideal for high-resolution, deep-tissue, time-lapse imaging of dynamic processes in cell biology. Here, we provide examples of important applications of 2PE for in vivo imaging of neuronal structure and signals; we also describe how it can be combined with optogenetics or photolysis of caged molecules to simultaneously probe and control neuronal activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.