ArticleMolecular biology reports2015
Selection of reference genes for quantitative real time RT-PCR during dimorphism in the zygomycete Mucor circinelloides.
Article in Molecular biology reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Functional characterization of two survival factor 1 genes inMicrobiology spectrum · 2024Article
- Blood Serum Stimulates the Virulence Potential of Mucorales through Enhancement in Mitochondrial Oxidative Metabolism and Rhizoferrin Production.Journal of fungi (Basel, Switzerland) · 2023Article
- Identification and evaluation of suitable reference genes for RT-qPCR analyses in Trichoderma atroviride under varying light conditions.Fungal biology and biotechnology · 2023Article
- Characterisation of putative class 1A DHODH-like proteins from Mucorales and dematiaceous mould species.PloS one · 2023Article
- Investigating the Effect of Alcohol Dehydrogenase Gene Knockout on Lipid Accumulation inJournal of fungi (Basel, Switzerland) · 2022Article
- Secretion of the siderophore rhizoferrin is regulated by the cAMP-PKA pathway and is involved in the virulence of Mucor lusitanicus.Scientific reports · 2022Article
- Differential Gene Expression ofJournal of fungi (Basel, Switzerland) · 2022Article
- Transcript pattern analysis of Arf-family genes in the phytopathogen Fusarium oxysporum f. sp. lycopersici reveals the role of Arl3 in the virulence.Antonie van Leeuwenhoek · 2021Article
- DNA Methylation on N6-Adenine Regulates the Hyphal Development during Dimorphism in the Early-Diverging FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Mass spore production of3 Biotech · 2021Article
- Dimorphic Mechanism on cAMP Mediated Signal Pathway in Mucor circinelloides.Applied biochemistry and biotechnology · 2021Article
- Alteration of Fermentative Metabolism Enhances Mucor circinelloides Virulence.Infection and immunity · 2020Article
- Alcohol dehydrogenase 1 participates in the Crabtree effect and connects fermentative and oxidative metabolism in the Zygomycete Mucor circinelloides.Journal of microbiology (Seoul, Korea) · 2019Article
- Article
- Control of morphology and virulence by ADP-ribosylation factors (Arf) in Mucor circinelloides.Current genetics · 2018Article
- Development of a plasmid free CRISPR-Cas9 system for the genetic modification of Mucor circinelloides.Scientific reports · 2017Article
- Article
- Identification and Evaluation of Reliable Reference Genes in the Medicinal Fungus Shiraia bambusicola.Current microbiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mucor circinelloides is a dimorphic fungal model for studying several biological processes including cell differentiation (yeast-mold transitions) as well as biodiesel and carotene production. The recent release of the first draft sequence of the M. circinelloides genome, combined with the availability of analytical methods to determine patterns of gene expression, such as quantitative Reverse transcription-Polymerase chain reaction (qRT-PCR), and the development of molecular genetic tools for the manipulation of the fungus, may help identify M. circinelloides gene products and analyze their relevance in different biological processes. However, no information is available on M. circinelloides genes of stable expression that could serve as internal references in qRT-PCR analyses. One approach to solve this problem consists in the use of housekeeping genes as internal references. However, validation of the usability of these reference genes is a fundamental step prior to initiating qRT-PCR assays. This work evaluates expression of several constitutive genes by qRT-PCR throughout the morphological differentiation stages of M. circinelloides; our results indicate that tfc-1 and ef-1 are the most stable genes for qRT-PCR assays during differentiation studies and they are proposed as reference genes to carry out gene expression studies in this fungus.
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