ArticleScientific reports2014
The choice of reference genes for assessing gene expression in sugarcane under salinity and drought stresses.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed, 125 citations in OpenAlex.
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- Development and selection of stably expressed reference genes for expression normalization in Ribes odoratum under drought stress.Scientific reports · 2025Article
- Selection of stable internal control genes for quantitative real-time PCR (RT-qPCR) in banana (Musa spp.) under short-term drought stress.Molecular biology reports · 2025Article
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- Selecting reference genes for RT-qPCR studies involving the presence of B chromosomes in Psalidodon (Characiformes, Characidae).Molecular biology reports · 2024Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Genome-wide analysis of the soybeanFrontiers in plant science · 2024Article
- Identification and characterization of theFrontiers in plant science · 2024Article
- Selection and validation of reliable reference genes for quantitative real-time PCR in Barnyard millet (Echinochloa spp.) under varied abiotic stress conditions.Scientific reports · 2023Article
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- A mulberry 9-Frontiers in plant science · 2023Article
- Transcriptome-wide selection and validation of a solid set of reference genes for gene expression studies in the cephalopod molluskFrontiers in molecular neuroscience · 2023Article
- SugarcaneInternational journal of molecular sciences · 2022Article
- Identification and Validation of Reliable Reference Genes for Gene Expression Studies inGenes · 2022Article
- Screening of stable internal reference gene of Quinoa under hormone treatment and abiotic stress.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
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- Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.).Scientific reports · 2021Article
- Dehydration-Induced WRKY Transcriptional Factor MfWRKY70 ofBiomolecules · 2021Article
- Selection of reference genes for normalization of microRNA expression in sugarcane stalks during its interaction with3 Biotech · 2021Article
3 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Sugarcane (Saccharum spp. hybrids) is a world-wide cash crop for sugar and biofuel in tropical and subtropical regions and suffers serious losses in cane yield and sugar content under salinity and drought stresses. Although real-time quantitative PCR has a numerous advantage in the expression quantification of stress-related genes for the elaboration of the corresponding molecular mechanism in sugarcane, the variation happened across the process of gene expression quantification should be normalized and monitored by introducing one or several reference genes. To validate suitable reference genes or gene sets for sugarcane gene expression normalization, 13 candidate reference genes have been tested across 12 NaCl- and PEG-treated sugarcane samples for four sugarcane genotypes using four commonly used systematic statistical algorithms termed geNorm, BestKeeper, NormFinder and the deltaCt method. The results demonstrated that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and eukaryotic elongation factor 1-alpha (eEF-1a) were identified as suitable reference genes for gene expression normalization under salinity/drought-treatment in sugarcane. Moreover, the expression analyses of SuSK and 6PGDH further validated that a combination of clathrin adaptor complex (CAC) and cullin (CUL) as reference should be better for gene expression normalization. These results can facilitate the future research on gene expression in sugarcane under salinity and drought stresses.
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