ArticlePloS one2014
Investigation of adhesion and mechanical properties of human glioma cells by single cell force spectroscopy and atomic force microscopy.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Mechanochemical interactions in cancer cells: The role of substrate stiffness in cell behavior and drug response.PloS one · 2026Article
- Recurrent Limitations of CAR-T Therapy in Gliomas: Evidence from Preclinical and Phase I Clinical Studies.International journal of molecular sciences · 2025Review
- Atomic Force Microscopy Reveals Differences In Mechanical Properties Linked To Cortical Structure In Mouse And Human Oocytes.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Exploring the heterogeneity in glioblastoma cellular mechanics using in-vitro assays and atomic force microscopy.Scientific reports · 2025Article
- Nanomechanical characterization of soft nanomaterial using atomic force microscopy.Materials today. Bio · 2025Review
- Machine learning and artificial intelligence: Enabling the clinical translation of atomic force microscopy-based biomarkers for cancer diagnosis.Computational and structural biotechnology journal · 2024Review
- Exosomal TNF-α mediates voltage-gated Na+ channel 1.6 overexpression and contributes to brain tumor-induced neuronal hyperexcitability.The Journal of clinical investigation · 2024Article
- Flagellar beating forces of human spermatozoa with different motility behaviors.Reproductive biology and endocrinology : RB&E · 2024Article
- Imaging the subcellular viscoelastic properties of mouse oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Quantifying Deformation and Migration Properties of U87 Glioma Cells Using Dielectrophoretic Forces.Biosensors · 2022Article
- The dual action of glioma-derived exosomes on neuronal activity: synchronization and disruption of synchrony.Cell death & disease · 2022Article
- Nanomechanical and Morphological AFM Mapping of Normal Tissues and Tumors on Live Brain Slices Using Specially Designed Embedding Matrix and Laser-Shaped Cantilevers.Biomedicines · 2022Article
- Microfabricated cantilevers for parallelized cell-cell adhesion measurements.European biophysics journal : EBJ · 2022Article
- Scanning Probe Microscopies: Imaging and Biomechanics in Reproductive Medicine Research.International journal of molecular sciences · 2021Review
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- Review
- Single-Cell Mechanophenotyping in Microfluidics to Evaluate Behavior of U87 Glioma Cells.Micromachines · 2020Article
- Application of atomic force microscope in diagnosis of single cancer cells.Biomicrofluidics · 2020Review
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Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Active cell migration and invasion is a peculiar feature of glioma that makes this tumor able to rapidly infiltrate into the surrounding brain tissue. In our recent work, we identified a novel class of glioma-associated-stem cells (defined as GASC for high-grade glioma--HG--and Gasc for low-grade glioma--LG) that, although not tumorigenic, act supporting the biological aggressiveness of glioma-initiating stem cells (defined as GSC for HG and Gsc for LG) favoring also their motility. Migrating cancer cells undergo considerable molecular and cellular changes by remodeling their cytoskeleton and cell interactions with surrounding environment. To get a better understanding about the role of the glioma-associated-stem cells in tumor progression, cell deformability and interactions between glioma-initiating stem cells and glioma-associated-stem cells were investigated. Adhesion of HG/LG-cancer cells on HG/LG-glioma-associated stem cells was studied by time-lapse microscopy, while cell deformability and cell-cell adhesion strengths were quantified by indentation measurements by atomic force microscopy and single cell force spectroscopy. Our results demonstrate that for both HG and LG glioma, cancer-initiating-stem cells are softer than glioma-associated-stem cells, in agreement with their neoplastic features. The adhesion strength of GSC on GASC appears to be significantly lower than that observed for Gsc on Gasc. Whereas, GSC spread and firmly adhere on Gasc with an adhesion strength increased as compared to that obtained on GASC. These findings highlight that the grade of glioma-associated-stem cells plays an important role in modulating cancer cell adhesion, which could affect glioma cell migration, invasion and thus cancer aggressiveness. Moreover this work provides evidence about the importance of investigating cell adhesion and elasticity for new developments in disease diagnostics and therapeutics.
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