ArticleNucleic acids research2014
Oxidative DNA damage is instrumental in hyperreplication stress-induced inviability of Escherichia coli.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- The loop domains of the E. coli β sliding clamp are required for Hda-dependent inactivation of DnaA.Genetics · 2026Article
- Transcription-replication collisions trigger high-fidelity replication reset.Nucleic acids research · 2025Article
- Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading.Nature communications · 2025Article
- Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria.Microbiology (Reading, England) · 2025Article
- Review
- Dispensability of extrinsic DnaA regulators inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Targeting synthesis of the Chromosome Replication Initiator Protein DnaA by antisense PNA-peptide conjugates inFrontiers in antibiotics · 2024Article
- Multiple mechanisms for overcoming lethal over-initiation of DNA replication.Molecular microbiology · 2022Article
- Escherichia coli induces DNA repair enzymes to protect itself from low-grade hydrogen peroxide stress.Molecular microbiology · 2022Article
- Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.Nucleic acids research · 2021Article
- Antisense inhibition of the Escherichia coli NrdAB aerobic ribonucleotide reductase is bactericidal due to induction of DNA strand breaks.The Journal of antimicrobial chemotherapy · 2021Article
- RecA Is Required for the Assembly of RecN into DNA Repair Complexes on the Nucleoid.Journal of bacteriology · 2021Article
- Aerobic Conditions and Endogenous Reactive Oxygen Species Reduce the Production of Infectious MS2 Phage byViruses · 2021Article
- Modulation ofFrontiers in genetics · 2020Article
- Linking high GC content to the repair of double strand breaks in prokaryotic genomes.PLoS genetics · 2019Article
- Genome-wide assessment of antimicrobial tolerance inMicrobial genomics · 2019Article
- Blocking the Trigger: Inhibition of the Initiation of Bacterial Chromosome Replication as an Antimicrobial Strategy.Antibiotics (Basel, Switzerland) · 2019Review
- Thymineless Death inJournal of bacteriology · 2019Article
- Review
- Incomplete base excision repair contributes to cell death from antibiotics and other stresses.DNA repair · 2018Review
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In Escherichia coli, an increase in the ATP bound form of the DnaA initiator protein results in hyperinitiation and inviability. Here, we show that such replication stress is tolerated during anaerobic growth. In hyperinitiating cells, a shift from anaerobic to aerobic growth resulted in appearance of fragmented chromosomes and a decrease in terminus concentration, leading to a dramatic increase in ori/ter ratio and cessation of cell growth. Aerobic viability was restored by reducing the level of reactive oxygen species (ROS) or by deleting mutM (Fpg glycosylase). The double-strand breaks observed in hyperinitiating cells therefore results from replication forks encountering single-stranded DNA lesions generated while removing oxidized bases, primarily 8-oxoG, from the DNA. We conclude that there is a delicate balance between chromosome replication and ROS inflicted DNA damage so the number of replication forks can only increase when ROS formation is reduced or when the pertinent repair is compromised.
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