ArticleNucleic acids research2014
Massively parallel determination and modeling of endonuclease substrate specificity.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Macromolecular modeling and design in Rosetta: recent methods and frameworks.Nature methods · 2020Review
- Modifying a covarying protein-DNA interaction changes substrate preference of a site-specific endonuclease.Nucleic acids research · 2019Article
- Deep sequencing methods for protein engineering and design.Current opinion in structural biology · 2017Review
- A human transcription factor in search mode.Nucleic acids research · 2016Article
- SMRT Sequencing for Parallel Analysis of Multiple Targets and Accurate SNP Phasing.G3 (Bethesda, Md.) · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We describe the identification and characterization of novel homing endonucleases using genome database mining to identify putative target sites, followed by high throughput activity screening in a bacterial selection system. We characterized the substrate specificity and kinetics of these endonucleases by monitoring DNA cleavage events with deep sequencing. The endonuclease specificities revealed by these experiments can be partially recapitulated using 3D structure-based computational models. Analysis of these models together with genome sequence data provide insights into how alternative endonuclease specificities were generated during natural evolution.
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Registered trials
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