ArticleMolecular & cellular proteomics : MCP2015
Glycoproteomic analysis of seven major allergenic proteins reveals novel post-translational modifications.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
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- Major Cat Allergen Fel d 4: Structure and Identification of a Cross-Reactive IgE-Epitope-Containing Area.Allergy · 2026Article
- Recent Advances in Nucleic Acid-Based Electrochemical Sensors for the Detection of Food Allergens.Sensors (Basel, Switzerland) · 2026Review
- An international seroprevalence survey of the IgE sensitisation to the Dermatophagoides farinae house dust mite and two of its major allergens (Der f 2, Zen 1) in atopic dogs.Veterinary dermatology · 2022Article
- Scientific Opinion on development needs for the allergenicity and protein safety assessment of food and feed products derived from biotechnology.EFSA journal. European Food Safety Authority · 2022Article
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- The Acari Hypothesis, II: Interspecies Operability of Pattern Recognition Receptors.Pathogens (Basel, Switzerland) · 2021Article
- The Major Peanut Allergen Ara h 2 Produced inFrontiers in plant science · 2021Article
- Plant ProteinFrontiers in plant science · 2021Review
- Subcutaneous immunotherapy with purified Der p1 and 2 suppresses type 2 immunity in a murine asthma model.Allergy · 2018Article
- Current (Food) Allergenic Risk Assessment: Is It Fit for Novel Foods? Status Quo and Identification of Gaps.Molecular nutrition & food research · 2018Review
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- N-glycan in cockroach allergen regulates human basophil function.Immunity, inflammation and disease · 2017Article
- Foodomics and Food Safety: Where We Are.Food technology and biotechnology · 2017Review
- Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD.Scientific reports · 2017Article
- Allergens of Blomia tropicalis: An Overview of Recombinant Molecules.International archives of allergy and immunology · 2017Review
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Allergenic proteins such as grass pollen and house dust mite (HDM) proteins are known to trigger hypersensitivity reactions of the immune system, leading to what is commonly known as allergy. Key allergenic proteins including sequence variants have been identified but characterization of their post-translational modifications (PTMs) is still limited. Here, we present a detailed PTM(1) characterization of a series of the main and clinically relevant allergens used in allergy tests and vaccines. We employ Orbitrap-based mass spectrometry with complementary fragmentation techniques (HCD/ETD) for site-specific PTM characterization by bottom-up analysis. In addition, top-down mass spectrometry is utilized for targeted analysis of individual proteins, revealing hitherto unknown PTMs of HDM allergens. We demonstrate the presence of lysine-linked polyhexose glycans and asparagine-linked N-acetylhexosamine glycans on HDM allergens. Moreover, we identified more complex glycan structures than previously reported on the major grass pollen group 1 and 5 allergens, implicating important roles for carbohydrates in allergen recognition and response by the immune system. The new findings are important for understanding basic disease-causing mechanisms at the cellular level, which ultimately may pave the way for instigating novel approaches for targeted desensitization strategies and improved allergy vaccines.
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