ArticleMolecular microbiology2015
CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- The protein-water H-bond network for bacterial response regulator REC domain activation.Journal of structural biology: X · 2026Article
- Acetylomics reveals an extensive acetylation diversity withinmicroLife · 2024Article
- Contribution of NmSystems · 2021Article
- Quantitative proteomics implicates YggT in streptomycin resistance in Salmonella enterica serovar Enteritidis.Biotechnology letters · 2021Article
- The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.The EMBO journal · 2021Article
- Allosteric Priming of E. coli CheY by the Flagellar Motor Protein FliM.Biophysical journal · 2020Article
- The Architectural Dynamics of the Bacterial Flagellar Motor Switch.Biomolecules · 2020Review
- Metabolic intermediate acetyl phosphate modulates bacterial virulence via acetylation.Emerging microbes & infections · 2019Article
- Perturbing the acetylation status of the Type IV pilus retraction motor, PilT, reduces Neisseria gonorrhoeae viability.Molecular microbiology · 2018Article
- Behavioral Variability and Phenotypic Diversity in Bacterial Chemotaxis.Annual review of biophysics · 2018Review
- Molecular dynamics simulation of bacterial flagella.Biophysical reviews · 2018Review
- Structure and function of the archaeal response regulator CheY.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Influence of Glucose Availability and CRP Acetylation on the Genome-Wide Transcriptional Response ofFrontiers in microbiology · 2018Article
- Involvement of Two-Component Signaling on Bacterial Motility and Biofilm Development.Journal of bacteriology · 2017Review
- Regulation, Function, and Detection of Protein Acetylation in Bacteria.Journal of bacteriology · 2017Review
- Bacterial protein acetylation: new discoveries unanswered questions.Current genetics · 2016Review
- The role of motility and chemotaxis in the bacterial colonization of protected surfaces.Scientific reports · 2016Article
- Protein acetylation dynamics in response to carbon overflow in Escherichia coli.Molecular microbiology · 2015Article
- Cross Talk Inhibition Nullified by a Receiver Domain Missense Substitution.Journal of bacteriology · 2015Article
- Flagellar region 3b supports strong expression of integrated DNA and the highest chromosomal integration efficiency of the Escherichia coli flagellar regions.Microbial biotechnology · 2015Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Stimulation of Escherichia coli with acetate elevates the acetylation level of the chemotaxis response regulator CheY. This elevation, in an unknown mechanism, activates CheY to generate clockwise rotation. Here, using quantitative selective reaction monitoring mass spectrometry and high-resolution targeted mass spectrometry, we identified K91 and K109 as the major sites whose acetylation level in vivo increases in response to acetate. Employing single and multiple lysine replacements in CheY, we found that K91 and K109 are also the sites mainly responsible for acetate-dependent clockwise generation. Furthermore, we showed that clockwise rotation is repressed when residue K91 is nonmodified, as evidenced by an increased ability of CheY to generate clockwise rotation when K91 was acetylated or replaced by specific amino acids. Using molecular dynamics simulations, we show that K91 repression is manifested in the conformational dynamics of the β4α4 loop, shifted toward an active state upon mutation. Removal of β4α4 loop repression may represent a general activation mechanism in CheY, pertaining also to the canonical phosphorylation activation pathway as suggested by crystal structures of active and inactive CheY from Thermotoga maritima. By way of elimination, we further suggest that K109 acetylation is actively involved in generating clockwise rotation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.