ArticleScientific reports2014
RhoA and membrane fluidity mediates the spatially polarized Src/FAK activation in response to shear stress.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
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- Mechanosignaling and 3D morphological adaptation of MSCs in response to hydrogel rigidity underpin angiogenic and immunomodulatory efficacy for ischemic injury regeneration.Bioactive materials · 2025Article
- Integrating molecular and cellular components of endothelial shear stress mechanotransduction.American journal of physiology. Heart and circulatory physiology · 2024Review
- Matrix stiffness, endothelial dysfunction and atherosclerosis.Molecular biology reports · 2023Review
- Vascular mechanotransduction.Physiological reviews · 2023Review
- High-Fat Mouse Model to Explore the Relationship between Abnormal Lipid Metabolism and Enolase in Pancreatic Cancer.Mediators of inflammation · 2023Article
- Frequency-dependent signaling in cardiac myocytes.Frontiers in physiology · 2022Article
- Molecular tension in syndecan-1 is regulated by extracellular mechanical cues and fluidic shear stress.Biomaterials · 2021Article
- Stent strut streamlining and thickness reduction promote endothelialization.Journal of the Royal Society, Interface · 2021Article
- Cell membrane rupture: a novel test reveals significant variations among different brands of tissue culture flasks.BMC research notes · 2021Article
- Maintenance of HDACs and H3K9me3 Prevents Arterial Flow-Induced Venous Endothelial Damage.Frontiers in cell and developmental biology · 2021Article
- Metabolic response of blood vessels to TNFα.eLife · 2020Article
- Heat Shock Protein 27 Phosphorylation Regulates Tumor Cell Migration under Shear Stress.Biomolecules · 2019Article
- Astrocyte Mechano-Activation by High-Rate Overpressure Involves Alterations in Structural and Junctional Proteins.Frontiers in neurology · 2019Article
- Functional impairment triggered by altertoxin II (ATXII) in intestinal cells in vitro: cross-talk between cytotoxicity and mechanotransduction.Archives of toxicology · 2018Article
- A Membrane-Bound Biosensor Visualizes Shear Stress-Induced Inhomogeneous Alteration of Cell Membrane Tension.iScience · 2018Article
- Src family kinases (SFKs) and cell polarity in the testis.Seminars in cell & developmental biology · 2018Review
- Role of membrane-tension gated CaJournal of cell science · 2018Article
- FRET biosensor allows spatio-temporal observation of shear stress-induced polar RhoGDIα activation.Communications biology · 2018Article
- Src-mediated tyrosine phosphorylation of Protein Kinase D2 at focal adhesions regulates cell adhesion.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
While Src plays crucial roles in shear stress-induced cellular processes, little is known on the spatiotemporal pattern of high shear stress (HSS)-induced Src activation. HSS (65 dyn/cm(2)) was applied on bovine aortic endothelial cells to visualize the dynamic Src activation at subcellular levels utilizing a membrane-targeted Src biosensor (Kras-Src) based on fluorescence resonance energy transfer (FRET). A polarized Src activation was observed with higher activity at the side facing the flow, which was enhanced by a cytochalasin D-mediated disruption of actin filaments but inhibited by a benzyl alcohol-mediated enhancement of membrane fluidity. Further experiments revealed that HSS decreased RhoA activity, with a constitutively active RhoA mutant inhibiting while a negative RhoA mutant enhancing the HSS-induced Src polarity. Cytochalasin D can restore the polarity in cells expressing the active RhoA mutant. Further results indicate that HSS stimulates FAK activation with a spatial polarity similar to Src. The inhibition of Src by PP1, as well as the perturbation of RhoA activity and membrane fluidity, can block this HSS-induced FAK polarity. These results indicate that the HSS-induced Src and subsequently FAK polarity depends on the coordination between intracellular tension distribution regulated by RhoA, its related actin structures and the plasma membrane fluidity.
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