ArticleThe Plant cell2014
RISAP is a TGN-associated RAC5 effector regulating membrane traffic during polar cell growth in tobacco.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Quantitatively Analyzing the Structural Organization at the Tip of Growing Tobacco Pollen Tubes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Essential developmental processes in Physcomitrium patens require distinct levels of total activity provided by functionally redundant PpROP GTPases.The New phytologist · 2025Article
- Polar localization and local translation of RHO-RELATED PROTEIN FROM PLANTS2 mRNAs promote root hair growth in Arabidopsis.The Plant cell · 2024Article
- A myosin XI adaptor, TAPE, is essential for pollen tube elongation in rice.Plant physiology · 2022Article
- Quantitative cell biology of tip growth in moss.Plant molecular biology · 2021Review
- Arabidopsis thaliana myosin XIK is recruited to the Golgi through interaction with a MyoB receptor.Communications biology · 2021Article
- Function of membrane domains in rho-of-plant signaling.Plant physiology · 2021Review
- Membrane imaging in the plant endomembrane system.Plant physiology · 2021Article
- Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies.PloS one · 2021Article
- Characterization of ancestral myosin XI from Marchantia polymorpha by heterologous expression in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
- Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth.Plants (Basel, Switzerland) · 2020Review
- Quantitative Structural Organization of Bulk Apical Membrane Traffic in Pollen Tubes.Plant physiology · 2020Article
- Control of the Actin Cytoskeleton Within Apical and Subapical Regions of Pollen Tubes.Frontiers in cell and developmental biology · 2020Review
- Exocytosis and Endocytosis: Yin-Yang Crosstalk for Sculpting a Dynamic Growing Pollen Tube Tip.Frontiers in plant science · 2020Review
- Rho-of-plant activated root hair formation requiresDevelopment (Cambridge, England) · 2019Article
- Analysis of the Localization of Fluorescent PpROP1 and PpROP-GEF4 Fusion Proteins in Moss Protonemata Based on Genomic "Knock-In" and Estradiol-Titratable Expression.Frontiers in plant science · 2019Article
- Kinesins and Myosins: Molecular Motors that Coordinate Cellular Functions in Plants.Annual review of plant biology · 2018Review
- The Actin Cytoskeleton: Functional Arrays for Cytoplasmic Organization and Cell Shape Control.Plant physiology · 2018Review
- Update on Myosin Motors: Molecular Mechanisms and Physiological Functions.Plant physiology · 2018Review
- ROP GTPases Structure-Function and Signaling Pathways.Plant physiology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RAC/ROP GTPases coordinate actin dynamics and membrane traffic during polar plant cell expansion. In tobacco (Nicotiana tabacum), pollen tube tip growth is controlled by the RAC/ROP GTPase RAC5, which specifically accumulates at the apical plasma membrane. Here, we describe the functional characterization of RISAP, a RAC5 effector identified by yeast (Saccharomyces cerevisiae) two-hybrid screening. RISAP belongs to a family of putative myosin receptors containing a domain of unknown function 593 (DUF593) and binds via its DUF593 to the globular tail domain of a tobacco pollen tube myosin XI. It also interacts with F-actin and is associated with a subapical trans-Golgi network (TGN) compartment, whose cytoplasmic position at the pollen tube tip is maintained by the actin cytoskeleton. In this TGN compartment, apical secretion and endocytic membrane recycling pathways required for tip growth appear to converge. RISAP overexpression interferes with apical membrane traffic and blocks tip growth. RAC5 constitutively binds to the N terminus of RISAP and interacts in an activation-dependent manner with the C-terminal half of this protein. In pollen tubes, interaction between RAC5 and RISAP is detectable at the subapical TGN compartment. We present a model of RISAP regulation and function that integrates all these findings.
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