ArticlePloS one2014
First TILLING platform in Cucurbita pepo: a new mutant resource for gene function and crop improvement.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata.Plant communications · 2026Article
- High throughput screening and evaluation of salt-tolerant mutants from an EMS collection ofFrontiers in plant science · 2025Article
- Review
- Genetic and Pre- and Postharvest Factors Influencing the Content of Antioxidants in Cucurbit Crops.Antioxidants (Basel, Switzerland) · 2021Review
- The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants.Frontiers in plant science · 2021Review
- Article
- Whole-genome resequencing ofHorticulture research · 2019Article
- Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING).BMC genomics · 2018Article
- Phenomic and Genomic Characterization of a Mutant Platform inFrontiers in plant science · 2018Article
- Article
- Induced Mutagenesis inFrontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the availability of genetic and genomic resources for Cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. In this direction, we have developed a high throughput reverse genetic tool: the first TILLING (Targeting Induced Local Lesions IN Genomes) resource for this species. Additionally, we have used this resource to demonstrate that the previous EMS mutant population we developed has the highest mutation density compared with other cucurbits mutant populations. The overall mutation density in this first C. pepo TILLING platform was estimated to be 1/133 Kb by screening five additional genes. In total, 58 mutations confirmed by sequencing were identified in the five targeted genes, thirteen of which were predicted to have an impact on the function of the protein. The genotype/phenotype correlation was studied in a peroxidase gene, revealing that the phenotype of seedling homozygous for one of the isolated mutant alleles was albino. These results indicate that the TILLING approach in this species was successful at providing new mutations and can address the major challenge of linking sequence information to biological function and also the identification of novel variation for crop breeding.
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