ArticleTheScientificWorldJournal2014
Rapid detection and differentiation of Clonorchis sinensis and Opisthorchis viverrini using real-time PCR and high resolution melting analysis.
Article in TheScientificWorldJournal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- High sensitivity detection ofFood science and biotechnology · 2025Article
- Current knowledge on the prevalence and detection techniques ofFrontiers in veterinary science · 2025Review
- Clonorchiasis and opisthorchiasis: epidemiology, transmission, clinical features, morbidity, diagnosis, treatment, and control.Clinical microbiology reviews · 2024Review
- Production and Immunological Characterization of scFv Specific to Epitope ofTropical medicine and infectious disease · 2023Article
- Efficiency of the Stool-PCR Test Targeting NADH Dehydrogenase (Journal of tropical medicine · 2021Article
- Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development.Parasitology · 2020Review
- High-Resolution Melting Analysis as an Appropriate Method to Differentiate betweenIranian journal of public health · 2019Article
- Performance evaluation of existing immunoassays for Clonorchis sinensis infection in China.Parasites & vectors · 2018Article
- Surveillance and diagnosis of zoonotic foodborne parasites.Food science & nutrition · 2018Review
- Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand.PLoS neglected tropical diseases · 2017Article
- Development of a PCR Assay for Diagnosing Trematode (Opisthorchis and Haplorchis) Infections in Human Stools.The American journal of tropical medicine and hygiene · 2017Article
- High-resolution melting analysis (HRM) for differentiation of four major Taeniidae species in dogs Taenia hydatigena, Taenia multiceps, Taenia ovis, and Echinococcus granulosus sensu stricto.Parasitology research · 2016Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clonorchis sinensis and Opisthorchis viverrini are both important fish-borne pathogens, causing serious public health problem in Asia. The present study developed an assay integrating real-time PCR and high resolution melting (HRM) analysis for the specific detection and rapid identification of C. sinensis and O. viverrini. Primers targeting COX1 gene were highly specific for these liver flukes, as evidenced by the negative amplification of closely related trematodes. Assays using genomic DNA extracted from the two flukes yielded specific amplification and their identity was confirmed by sequencing, having the accuracy of 100% in reference to conventional methods. The assay was proved to be highly sensitive with a detection limit below 1 pg of purified genomic DNA, 5 EPG, or 1 metacercaria of C. sinensis. Moreover, C. sinensis and O. viverrini were able to be differentiated by their HRM profiles. The method can reduce labor of microscopic examination and the contamination of agarose electrophoresis. Moreover, it can differentiate these two flukes which are difficult to be distinguished using other methods. The established method provides an alternative tool for rapid, simple, and duplex detection of C. sinensis and O. viverrini.
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