ArticleInfection and immunity2015
Prolonged growth of a clinical Staphylococcus aureus strain selects for a stable small-colony-variant cell type.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
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- Integrative omics analysis reveals insights into small colony variants of Staphylococcus aureus induced by sulfamethoxazole-trimethoprim.BMC microbiology · 2024Article
- Generation and Characterization of Stable Small Colony Variants of USA300Antibiotics (Basel, Switzerland) · 2024Article
- Inulin-lipid hybrid (ILH) microparticles promote pH-triggered release of rifampicin within infected macrophages.Drug delivery and translational research · 2023Article
- Mesoporous Organosilica Nanoparticles to Fight Intracellular Staphylococcal Aureus Infections in Macrophages.Pharmaceutics · 2023Article
- Subpopulations in Strains ofAntibiotics (Basel, Switzerland) · 2023Review
- The first genomic characterization of a stable, hemin-dependent small colony variant strain ofFrontiers in microbiology · 2023Article
- Review on skeletal disorders caused byThe veterinary quarterly · 2022Review
- FunctionalJournal of bacteriology · 2022Article
- Iron restriction induces the small-colony variant phenotype inFrontiers in microbiology · 2022Article
- The road to success of coagulase-negative staphylococci: clinical significance of small colony variants and their pathogenic role in persistent infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Review
- A functional menadione biosynthesis pathway is required for capsule production byMicrobiology (Reading, England) · 2021Article
- Adaptation ofToxins · 2021Article
- A Human Osteocyte Cell Line Model for StudyingFrontiers in cellular and infection microbiology · 2021Article
- Novel Research Models forFrontiers in microbiology · 2020Review
- Modeling Early Stages of Bone and Joint Infections Dynamics in Humans: A Multi-Agent, Multi-System Based Model.Frontiers in molecular biosciences · 2020Article
- Cigarette smoke exposure redirects Staphylococcus aureus to a virulence profile associated with persistent infection.Scientific reports · 2019Article
- Rifampicin-Loaded Mesoporous Silica Nanoparticles for the Treatment of Intracellular Infections.Antibiotics (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An undetermined feature of Staphylococcus aureus pathogenesis is its persistence and then relapse of disease. This has been explained by its switch to alternative lifestyles, mainly as biofilm or small-colony variants (SCVs). Studying the native characteristics of SCVs has been problematic due to their reversion to the parental lifestyle. We have observed that for a number of S. aureus strains as they switch to an SCV lifestyle, there is the formation of an extracellular matrix. We focused our analysis on one strain, WCH-SK2. For bacterial survival in the host, the combination of low nutrients and the prolonged time frame forms a stress that selects for a specific cell type from the population. In this context, we used steady-state growth conditions with low nutrients and a controlled low growth rate for a prolonged time and with methylglyoxal. These conditions induced S. aureus WCH-SK2 into a stable SCV cell type; the cells did not revert after subculturing. Analysis revealed these cells possessed a metabolic and surface profile that was different from those of previously described SCVs or biofilm cells. The extracellular matrix was protein and extracellular DNA but not polysaccharide. The SCV cells induced expression of certain surface proteins (such as Ebh) and synthesis of lantibiotics while downregulating factors that stimulate the immune response (leucocidin, capsule, and carotenoid). Our data reveal cell heterogeneity within an S. aureus population and under conditions that resemble long-term survival in the host have identified a previously unnoticed S. aureus cell type with a distinctive metabolic and molecular profile.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.