ArticleInfection and immunity2015
Enterococcus faecalis 6-phosphogluconolactonase is required for both commensal and pathogenic interactions with Manduca sexta.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
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- Sortase-encoding genes,Frontiers in microbiology · 2024Article
- Host's genetic background determines the outcome of reciprocal faecal transplantation on life-history traits and microbiome composition.Animal microbiome · 2022Article
- A previously uncharacterized gene, PA2146, contributes to biofilm formation and drug tolerance across the ɣ-Proteobacteria.NPJ biofilms and microbiomes · 2022Article
- All Driven by Energy Demand? Integrative Comparison of Metabolism of Enterococcus faecalis Wildtype and a Glutamine Synthase Mutant.Microbiology spectrum · 2022Article
- Interactions between Blastocystis subtype ST4 and gut microbiota in vitro.Parasites & vectors · 2022Article
- Variability of Gut Microbiota Across the Life Cycle ofFrontiers in microbiology · 2020Article
- Antimicrobial resistance and genetic relationships of enterococci from siblings and non-siblingsPeerJ · 2020Article
- Interactions between a pathogenic Blastocystis subtype and gut microbiota: in vitro and in vivo studies.Microbiome · 2019Article
- Article
- Exploiting biofilm phenotypes for functional characterization of hypothetical genes inNPJ biofilms and microbiomesArticle
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Enterococcus faecalis is a commensal and pathogen of humans and insects. In Manduca sexta, E. faecalis is an infrequent member of the commensal gut community, but its translocation to the hemocoel results in a commensal-to-pathogen switch. To investigate E. faecalis factors required for commensalism, we identified E. faecalis genes that are upregulated in the gut of M. sexta using recombinase-based in vivo expression technology (RIVET). The RIVET screen produced 113 clones, from which we identified 50 genes that are more highly expressed in the insect gut than in culture. The most frequently recovered gene was locus OG1RF_11582, which encodes a 6-phosphogluconolactonase that we designated pglA. A pglA deletion mutant was impaired in both pathogenesis and gut persistence in M. sexta and produced enhanced biofilms compared with the wild type in an in vitro polystyrene plate assay. Mutation of four other genes identified by RIVET did not affect persistence in caterpillar guts but led to impaired pathogenesis. This is the first identification of genetic determinants for E. faecalis commensal and pathogenic interactions with M. sexta. Bacterial factors identified in this model system may provide insight into colonization or persistence in other host-associated microbial communities and represent potential targets for interventions to prevent E. faecalis infections.
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