ArticleJournal of clinical oncology : official journal of the American Society of Clinical Oncology2014
Identification of independent primary tumors and intrapulmonary metastases using DNA rearrangements in non-small-cell lung cancer.
Article in Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 2 of them syntheses that pooled it.
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74 citing papers in PubMed, 2 syntheses or guidelines pooled it, 131 citations in OpenAlex.
- Evaluation of surgical outcomes and prognostic factors of second primary lung cancer based on a systematic review and meta-analysis.BMC surgery · 2023Pooled it
- Surgical treatment to multiple primary lung cancer patients: a systematic review and meta-analysis.BMC surgery · 2019Pooled it
- Multiple Primary Lung Cancers in Surgical Patients: Revisiting Martini and Melamed 50 Years Later.Cancers · 2026Article
- The Added Value of Genome-Wide Copy Numbers to Objectively Resolve Clonality of Multiple Tumors With Pulmonary Involvement and Ambiguous or Inconclusive Mutational Diagnosis.JTO clinical and research reports · 2025Article
- The characteristics and prognoses of 72 postoperative synchronous multiple primary lung cancer patients.World journal of surgical oncology · 2025Article
- Lung metastases.Nature reviews. Disease primers · 2025Review
- Co-existence of Primary Glioma and Lymphoma: An Unusual Case Report.NMC case report journal · 2025Article
- Single-cell dissection reveals immunosuppressive F13A1+ macrophage as a hallmark for multiple primary lung cancers.Clinical and translational medicine · 2024Article
- The characteristics and prognosis of different disease patterns of multiple primary lung cancers categorized according to the 8th edition lung cancer staging system.Journal of cardiothoracic surgery · 2024Article
- Long-Term Survival and CANARY-Based Artificial Intelligence for Multifocal Lung Adenocarcinoma.Mayo Clinic proceedings. Digital health · 2024Article
- Mutually exclusive driver mutations identifies 2 separate primaries in a collision tumor initially interpreted as a solitary lung adenocarcinoma with tumor heterogeneity.Respiratory medicine case reports · 2024Article
- Diagnosis and management of multiple primary lung cancer.Frontiers in oncology · 2024Review
- Genomic heterogeneity of multiple synchronous lung cancers in Chinese population.Cancer medicine · 2024Article
- Lobar or sublobar resection for early-stage second primary lung cancer ≤ 3 cm in size: a SEER population-based study.Journal of cancer research and clinical oncology · 2023Article
- A pairwise radiomics algorithm-lesion pair relation estimation model for distinguishing multiple primary lung cancer from intrapulmonary metastasis.Precision clinical medicine · 2023Article
- Single-cell RNA sequencing analysis reveals transcriptional heterogeneity of multiple primary lung cancer.Clinical and translational medicine · 2023Article
- Case report: targeted sequencing improves the diagnosis of multiple synchronous lung cancers.Translational lung cancer research · 2023Article
- Genetic trajectory and clonal evolution of multiple primary lung cancer with lymph node metastasis.Cancer gene therapy · 2023Article
- Mate Pair Sequencing: Next-Generation Sequencing for Structural Variant Detection.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Lineage tracing for multiple lung cancer by spatiotemporal heterogeneity using a multi-omics analysis method integrating genomic, transcriptomic, and immune-related features.Frontiers in oncology · 2023Article
14 more citing papers are in PubMed but not listed here.
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14 authors at 3 institutions in 1 country.
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Abstract
purposeDistinguishing independent primary tumors from intrapulmonary metastases in non-small-cell carcinoma remains a clinical dilemma with significant clinical implications. Using next-generation DNA sequencing, we developed a chromosomal rearrangement-based approach to differentiate multiple primary tumors from metastasis.
methodsTumor specimens from patients with known independent primary tumors and metastatic lesions were used for lineage test development, which was then applied to multifocal tumors. Laser capture microdissection was performed separately for each tumor. Genomic DNA was isolated using direct in situ whole-genome amplification methodology, and next-generation sequencing was performed using an Illumina mate-pair library protocol. Sequence reads were mapped to the human genome, and primers spanning the fusion junctions were used for validation polymerase chain reaction.
resultsA total of 41 tumor samples were sequenced (33 adenocarcinomas [ADs] and eight squamous cell carcinomas [SQCCs]), with a range of three to 276 breakpoints per tumor identified. Lung tumors predicted to be independent primary tumors based on different histologic subtype did not share any genomic rearrangements. In patients with lung primary tumors and paired distant metastases, shared rearrangements were identified in all tumor pairs, emphasizing the patient specificity of identified breakpoints. Multifocal AD and SQCC samples were reviewed independently by two pulmonary pathologists. Concordance between histology and genomic data occurred in the majority of samples. Discrepant tumor samples were resolved by genome sequencing.
conclusionA diagnostic lineage test based on genomic rearrangements from mate-pair sequencing demonstrates promise for distinguishing independent primary from metastatic disease in lung cancer.
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