ArticleReproduction (Cambridge, England)2015
Requirement of the transcription factor USF1 in bovine oocyte and early embryonic development.
Article in Reproduction (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Transcription Factor TCF12-Mediated Maternal Gene Expressions in Mouse Oocyte Are Prerequisites of Successful Fertilisation and Zygotic Genome Activation.Cell proliferation · 2026Article
- Genome-wide association analysis highlights genomic regions and genes potentially associated with anestrus in crossbred gilts.Mammalian genome : official journal of the International Mammalian Genome Society · 2025Article
- CRISPR/Cas9-mediated deletion of a GA-repeat in human GPM6B leads to disruption of neural cell differentiation from NT2 cells.Scientific reports · 2024Article
- The Role of BDNF, YBX1, CENPF, ZSCAN4, TEAD4, GLIS1 and USF1 in the Activation of the Embryonic Genome in Bovine Embryos.International journal of molecular sciences · 2023Review
- The Dynamic Changes of Transcription Factors During the Development Processes of Human Biparental and Uniparental Embryos.Frontiers in cell and developmental biology · 2021Article
- Embryonic MTHFR contributes to blastocyst development.Journal of assisted reproduction and genetics · 2020Article
- Role of bone morphogenetic protein signaling in bovine early embryonic development and stage specific embryotropic actions of follistatin†.Biology of reproduction · 2020Article
- Extracellular Vesicles Function as Bioactive Molecular Transmitters in the Mammalian Oviduct: An Inspiration for Optimizing in Vitro Culture Systems and Improving Delivery of Exogenous Nucleic Acids during Preimplantation Embryonic Development.International journal of molecular sciences · 2020Review
- DNA methylation and functional characterization of the XIST gene duringEpigenetics · 2019Article
- Lipofection of siRNA into bovine 8-16-cell stage embryos using zona removal and the well-of-the-well culture system.The Journal of reproduction and development · 2018Article
- Sperm-borne miR-449b influences cleavage, epigenetic reprogramming and apoptosis of SCNT embryos in bovine.Scientific reports · 2017Article
- Genome-wide identification, classification, and functional analysis of the basic helix-loop-helix transcription factors in the cattle, Bos Taurus.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Upstream stimulating factor 1 (USF1) is a basic helix-loop-helix transcription factor that specifically binds to E-box DNA motifs, known cis-elements of key oocyte expressed genes essential for oocyte and early embryonic development. However, the functional and regulatory role of USF1 in bovine oocyte and embryo development is not understood. In this study, we demonstrated that USF1 mRNA is maternal in origin and expressed in a stage specific manner during the course of oocyte maturation and preimplantation embryonic development. Immunocytochemical analysis showed detectable USF1 protein during oocyte maturation and early embryonic development with increased abundance at 8-16-cell stage of embryo development, suggesting a potential role in embryonic genome activation. Knockdown of USF1 in germinal vesicle stage oocytes did not affect meiotic maturation or cumulus expansion, but caused significant changes in mRNA abundance for genes associated with oocyte developmental competence. Furthermore, siRNA-mediated depletion of USF1 in presumptive zygote stage embryos demonstrated that USF1 is required for early embryonic development to the blastocyst stage. A similar (USF2) yet unique (TWIST2) expression pattern during oocyte and early embryonic development for related E-box binding transcription factors known to cooperatively bind USF1 implies a potential link to USF1 action. This study demonstrates that USF1 is a maternally derived transcription factor required for bovine early embryonic development, which also functions in regulation of JY1, GDF9, and FST genes associated with oocyte competence.
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