ArticleProceedings of the National Academy of Sciences of the United States of America2014
Single mutations in sasA enable a simpler ΔcikA gene network architecture with equivalent circadian properties.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- The circadian clock and darkness control natural competence in cyanobacteria.Nature communications · 2020Article
- Minimal tool set for a prokaryotic circadian clock.BMC evolutionary biology · 2017Article
- A Combined Computational and Genetic Approach Uncovers Network Interactions of the Cyanobacterial Circadian Clock.Journal of bacteriology · 2016Article
- A dynamic interaction process between KaiA and KaiC is critical to the cyanobacterial circadian oscillator.Scientific reports · 2016Article
- Discrete gene replication events drive coupling between the cell cycle and circadian clocks.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Circadian Rhythms in Cyanobacteria.Microbiology and molecular biology reviews : MMBR · 2015Review
- Giving Time Purpose: The Synechococcus elongatus Clock in a Broader Network Context.Annual review of genetics · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The circadian input kinase of the cyanobacterium Synechococcus elongatus PCC 7942 (CikA) is important both for synchronizing circadian rhythms with external environmental cycles and for transferring temporal information between the oscillator and the global transcriptional regulator RpaA (regulator of phycobilisome-associated A). KOs of cikA result in one of the most severely altered but still rhythmic circadian phenotypes observed. We chemically mutagenized a cikA-null S. elongatus strain and screened for second-site suppressor mutations that could restore normal circadian rhythms. We identified two independent mutations in the Synechococcus adaptive sensor A (sasA) gene that produce nearly WT rhythms of gene expression, likely because they compensate for the loss of CikA on the temporal phosphorylation of RpaA. Additionally, these mutations restore the ability to reset the clock after a short dark pulse through an output-independent pathway, suggesting that SasA can influence entrainment through direct interactions with KaiC, a property previously unattributed to it. These experiments question the evolutionary advantage of integrating CikA into the cyanobacterial clock, challenge the conventional construct of separable input and output pathways, and show how easily the cell can adapt to restore phenotype in a severely compromised genetic network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.