ArticleProceedings of the National Academy of Sciences of the United States of America2014
Cross-species genetic exchange between visceral and cutaneous strains of Leishmania in the sand fly vector.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 93 citations in OpenAlex.
- Extensive heterozygosity and genetic exchange among natural populations ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Genomic instability and mono-parental expression mitigate genomic shock in a cross-subgenusNAR molecular medicine · 2026Article
- Epidemiological dynamics ofMicrobial genomics · 2025Article
- Unraveling the Genome Diversity ofLife (Basel, Switzerland) · 2025Review
- Cross-subgenus hybridization betweenbioRxiv : the preprint server for biology · 2025Article
- The impact of blood on vector-borne diseases with emphasis on mosquitoes and sand flies.Trends in parasitology · 2025Review
- BALB/c mice infection with hybridFrontiers in cellular and infection microbiology · 2025Article
- Spatial prediction of canine visceral leishmaniasis in an endemic urban area of Brazil.PloS one · 2025Article
- Understanding the genetic complexity of Leishmania infantum in the Americas: a focus on 3'NT/NU gene deletion.Memorias do Instituto Oswaldo Cruz · 2025Review
- A novel strain of Leishmania braziliensis harbors not a toti- but a bunyavirus.PLoS neglected tropical diseases · 2024Article
- Article
- Overview and Approaches for Handling of Animal Models of Leishmaniasis.Comparative medicine · 2024Review
- Extant interspecific hybridization among trematodes within the Schistosoma haematobium species complex in Nigeria.PLoS neglected tropical diseases · 2024Article
- HOP1 and HAP2 are conserved components of the meiosis-related machinery required for successful mating in Leishmania.Nature communications · 2023Article
- Article
- First evidence of experimental genetic hybridization between cutaneous and visceral strains of Leishmania donovani within its natural vector Phlebotomus argentipes.Acta tropica · 2023Article
- Article
- Life in plastic, it's fantastic! HowFrontiers in cellular and infection microbiology · 2023Review
- Self-Hybridization in Leishmania major.mBio · 2022Article
- Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods.Parasites & vectors · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
Genetic exchange between Leishmania major strains during their development in the sand fly vector has been experimentally shown. To investigate the possibility of genetic exchange between different Leishmania species, a cutaneous strain of L. major and a visceral strain of Leishmania infantum, each bearing a different drug-resistant marker, were used to coinfect Lutzomyia longipalpis sand flies. Eleven double-drug-resistant progeny clones, each the product of an independent mating event, were generated and submitted to genotype and phenotype analyses. The analysis of multiple allelic markers across the genome suggested that each progeny clone inherited at least one full set of chromosomes from each parent, with loss of heterozygosity at some loci, and uniparental retention of maxicircle kinetoplast DNA. Hybrids with DNA contents of approximately 2n, 3n, and 4n were observed. In vivo studies revealed clear differences in the ability of the hybrids to produce pathology in the skin or to disseminate to and grow in the viscera, suggesting polymorphisms and differential inheritance of the gene(s) controlling these traits. The studies, to our knowledge, represent the first experimental confirmation of cross-species mating in Leishmania, opening the way toward genetic linkage analysis of important traits and providing strong evidence that genetic exchange is responsible for the generation of the mixed-species genotypes observed in natural populations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.