ArticleBMC biology2014
Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission.
Article in BMC biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Review
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- Progress in cancer neuroscience.MedComm · 2023Review
- The neuroscience of cancer.Nature · 2023Review
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- Sensory axons induce epithelial lipid microdomain remodeling and determine the distribution of junctions in the epidermis.Molecular biology of the cell · 2023Article
- SOX2 and SOX21 in Lung Epithelial Differentiation and Repair.International journal of molecular sciences · 2022Review
- Innervation in organogenesis.Current topics in developmental biology · 2022Article
- Conserved Mechanisms in the Formation of the Airways and Alveoli of the Lung.Frontiers in cell and developmental biology · 2021Article
- Tumour innervation and neurosignalling in prostate cancer.Nature reviews. Urology · 2020Review
- The Lung Vasculature: A Driver or Passenger in Lung Branching Morphogenesis?Frontiers in cell and developmental biology · 2020Review
- Fgf10/Fgfr2b Signaling Orchestrates the Symphony of Molecular, Cellular, and Physical Processes Required for Harmonious Airway Branching Morphogenesis.Frontiers in cell and developmental biology · 2020Review
- Asthma: A Loss of Post-natal Homeostatic Control of Airways Smooth Muscle With Regression Toward a Pre-natal State.Frontiers in pediatrics · 2020Review
- Airway Innervation and Plasticity in Asthma.Physiology (Bethesda, Md.) · 2019Review
- SERCA directs cell migration and branching across species and germ layers.Biology open · 2017Article
- In Vitro Models to Study Human Lung Development, Disease and Homeostasis.Physiology (Bethesda, Md.) · 2017Review
- Patterned cell and matrix dynamics in branching morphogenesis.The Journal of cell biology · 2017Review
- Article
- How to Grow a Lung: Applying Principles of Developmental Biology to Generate Lung Lineages from Human Pluripotent Stem Cells.Current pathobiology reportsReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundParasympathetic signaling has been inferred to regulate epithelial branching as well as organ regeneration and tumor development. However, the relative contribution of local nerve contact versus secreted signals remains unclear. Here, we show a conserved (vertebrates to invertebrates) requirement for intact local nerves in airway branching, persisting even when cholinergic neurotransmission is blocked.
resultsIn the vertebrate lung, deleting enhanced green fluorescent protein (eGFP)-labeled intrinsic neurons using a two-photon laser leaves adjacent cells intact, but abolishes branching. Branching is unaffected by similar laser power delivered to the immediately adjacent non-neural mesodermal tissue, by blocking cholinergic receptors or by blocking synaptic transmission with botulinum toxin A. Because adjacent vasculature and epithelial proliferation also contribute to branching in the vertebrate lung, the direct dependence on nerves for airway branching was tested by deleting neurons in Drosophila embryos. A specific deletion of neurons in the Drosophila embryo by driving cell-autonomous RicinA under the pan-neuronal elav enhancer perturbed Drosophila airway development. This system confirmed that even in the absence of a vasculature or epithelial proliferation, airway branching is still disrupted by neural lesioning.
conclusionsTogether, this shows that airway morphogenesis requires local innervation in vertebrates and invertebrates, yet neurotransmission is dispensable. The need for innervation persists in the fly, wherein adjacent vasculature and epithelial proliferation are absent. Our novel, targeted laser ablation technique permitted the local function of parasympathetic innervation to be distinguished from neurotransmission.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.