ArticleMolecular plant pathology2015
The c-di-GMP phosphodiesterase BifA is involved in the virulence of bacteria from the Pseudomonas syringae complex.
Article in Molecular plant pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 34 citations in OpenAlex.
- AraC-Family Transcriptional Regulator WhpR Controls Virulence in Pseudomonas savastanoi pv. savastanoi Through Regulation of Indole Metabolism.Microbial biotechnology · 2025Article
- VimR is a conserved master transcription regulator controlling virulence and metabolism in Pseudomonas.Nucleic acids research · 2025Article
- Fostering plant protection against certain bacterial diseases through quorum-sensing signal molecules: a critical review.Frontiers in plant science · 2025Review
- Article
- Origins of symbiosis: shared mechanisms underlying microbial pathogenesis, commensalism and mutualism of plants and animals.FEMS microbiology reviews · 2023Review
- Motility, Adhesion and c-di-GMP Influence the Endophytic Colonization of Rice byMicroorganisms · 2021Article
- Signal transduction schemes inComputational and structural biotechnology journal · 2020Review
- GenesFrontiers in plant science · 2020Article
- Signal Sensing and Transduction Are Conserved between the Periplasmic Sensory Domains of BifA and SagS.mSphere · 2019Article
- Pleiotropic Effects of c-di-GMP Content inApplied and environmental microbiology · 2019Article
- Phosphodiesterase Genes Regulate Amylovoran Production, Biofilm Formation, and Virulence in Erwinia amylovora.Applied and environmental microbiology · 2019Article
- Article
- c-di-GMP Regulates Various Phenotypes and Insecticidal Activity of Gram-PositiveFrontiers in microbiology · 2018Article
- Knots Untie: Molecular Determinants Involved in Knot Formation Induced byFrontiers in plant science · 2017Review
- High levels of cyclic-di-GMP in plant-associated Pseudomonas correlate with evasion of plant immunity.Molecular plant pathology · 2016Article
- Bacterial rotary export ATPases are allosterically regulated by the nucleotide second messenger cyclic-di-GMP.The Journal of biological chemistry · 2015Article
- The olive knot disease as a model to study the role of interspecies bacterial communities in plant disease.Frontiers in plant science · 2015Review
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Authors and funding
4 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In a recent screen for novel virulence factors involved in the interaction between Pseudomonas savastanoi pv. savastanoi and the olive tree, a mutant was selected that contained a transposon insertion in a putative cyclic diguanylate (c-di-GMP) phosphodiesterase-encoding gene. This gene displayed high similarity to bifA of Pseudomonas aeruginosa and Pseudomonas putida. Here, we examined the role of BifA in free-living and virulence-related phenotypes of two bacterial plant pathogens in the Pseudomonas syringae complex, the tumour-inducing pathogen of woody hosts, P. savastanoi pv. savastanoi NCPPB 3335, and the pathogen of tomato and Arabidopsis, P. syringae pv. tomato DC3000. We showed that deletion of the bifA gene resulted in decreased swimming motility of both bacteria and inhibited swarming motility of DC3000. In contrast, overexpression of BifA in P. savastanoi pv. savastanoi had a positive impact on swimming motility and negatively affected biofilm formation. Deletion of bifA in NCPPB 3335 and DC3000 resulted in reduced fitness and virulence of the microbes in olive (NCPPB 3335) and tomato (DC3000) plants. In addition, real-time monitoring of olive plants infected with green fluorescent protein (GFP)-tagged P. savastanoi cells displayed an altered spatial distribution of mutant ΔbifA cells inside olive knots compared with the wild-type strain. All free-living phenotypes that were altered in both ΔbifA mutants, as well as the virulence of the NCPPB 3335 ΔbifA mutant in olive plants, were fully rescued by complementation with P. aeruginosa BifA, whose phosphodiesterase activity has been demonstrated. Thus, these results suggest that P. syringae and P. savastanoi BifA are also active phosphodiesterases. This first demonstration of the involvement of a putative phosphodiesterase in the virulence of the P. syringae complex provides confirmation of the role of c-di-GMP signalling in the virulence of this group of plant pathogens.
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