ArticleChromosoma2015
Deletion of Drosophila Nopp140 induces subcellular ribosomopathies.
Article in Chromosoma, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Genetic Factors Linking Nucleolar Stress withInternational journal of molecular sciences · 2025Article
- Insulin signaling regulates R2 retrotransposon expression to orchestrate transgenerational rDNA copy number maintenance.Nature communications · 2025Article
- Comprehensive map of ribosomal 2'-O-methylation and C/D box snoRNAs in Drosophila melanogaster.Nucleic acids research · 2024Article
- An RNAi screen for ribosome biogenesis genes required formicroPublication biology · 2024Article
- The retrotransposon R2 maintainsProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Under the magnifying glass: The ups and downs of rDNA copy number.Seminars in cell & developmental biology · 2023Review
- A clinically-relevant residue of POLR1D is required for Drosophila development.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon.Nucleic acids research · 2022Article
- Nopp140-chaperoned 2'-O-methylation of small nuclear RNAs in Cajal bodies ensures splicing fidelity.Genes & development · 2021Article
- Article
- Nucleolar stress inBiology open · 2020Article
- The Nopp140 gene in Drosophila melanogaster displays length polymorphisms in its large repetitive second exon.Molecular genetics and genomics : MGG · 2019Article
- The nucleolar transcriptome regulates Piwi shuttling between the nucleolus and the nucleoplasm.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Article
- R1 retrotransposons in the nucleolar organizers of Drosophila melanogaster are transcribed by RNA polymerase I upon heat shock.Transcription · 2018Article
- Regulatory RNPs: a novel class of ribonucleoproteins that potentially contribute to ribosome heterogeneity.Biology open · 2017Article
- Juvenile hormone and its receptor methoprene-tolerant promote ribosomal biogenesis and vitellogenesis in theThe Journal of biological chemistry · 2017Article
- Towards an understanding of regulating Cajal body activity by protein modification.RNA biology · 2017Review
- Loss of Drosophila nucleostemin 2 (NS2) blocks nucleolar release of the 60S subunit leading to ribosome stress.Chromosoma · 2017Article
- TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands.Open biology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleolar and Cajal body phosphoprotein of 140 kDa (Nopp140) is considered a ribosome assembly factor, but its precise functions remain unknown. To approach this problem, we deleted the Nopp140 gene in Drosophila using FLP-FRT recombination. Genomic PCR, reverse transcriptase-PCR (RT-PCR), and immunofluorescence microscopy confirmed the loss of Nopp140, its messenger RNA (mRNA), and protein products from all tissues examined. Nopp140-/- larvae arrested in the second instar stage and most died within 8 days. While nucleoli appeared intact in Nopp140-/- cells, the C/D small nucleolar ribonucleoprotein (snoRNP) methyltransferase, fibrillarin, redistributed to the nucleoplasm in variable amounts depending on the cell type; RT-PCRs showed that 2'-O-methylation of ribosomal RNA (rRNA) in Nopp140-/- cells was reduced at select sites within both the 18S and 28S rRNAs. Ultrastructural analysis showed that Nopp140-/- cells were deficient in cytoplasmic ribosomes, but instead contained abnormal electron-dense cytoplasmic granules. Immunoblot analysis showed a loss of RpL34, and metabolic labeling showed a significant drop in protein translation, supporting the loss of functional ribosomes. Northern blots showed that pre-RNA cleavage pathways were generally unaffected by the loss of Nopp140, but that R2 retrotransposons that naturally reside within the 28S region of normally silent heterochromatic Drosophila ribosomal DNA (rDNA) genes were selectively expressed in Nopp140-/- larvae. Unlike copia elements and the related R1 retrotransposon, R2 expression appeared to be preferentially dependent on the loss of Nopp140 and not on environmental stresses. We believe the phenotypes described here define novel intracellular ribosomopathies resulting from the loss of Nopp140.
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