ArticleThe Journal of cell biology1989
Visualization of cyclic nucleotide binding sites in the vertebrate retina by fluorescence microscopy.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 29 citations in OpenAlex.
- Protein Kinase A Catalytic and Regulatory Subunits Interact Differently in Various Areas of Mouse Brain.International journal of molecular sciences · 2020Article
- Protein Kinase A Distribution in Meningioma.Cancers · 2019Article
- Organization of cGMP sensing structures on the rod photoreceptor outer segment plasma membrane.Channels (Austin, Tex.) · 2014Article
- Myo3A, one of two class III myosin genes expressed in vertebrate retina, is localized to the calycal processes of rod and cone photoreceptors and is expressed in the sacculus.Molecular biology of the cell · 2003Article
- Cyclic GMP-activated channels of salamander retinal rods: spatial distribution and variation of responsiveness.The Journal of physiology · 1992Article
- Association of cyclic GMP-gated channels and Na(+)-Ca(2+)-K+ exchangers in bovine retinal rod outer segment plasma membranes.The Journal of physiology · 1992Article
- Diltiazem at high concentration increases the ionic permeability of biological membranes.The Journal of membrane biology · 1991Article
- Guanosine 3',5'-cyclic nucleotide binding proteins of bovine retina identified by photoaffinity labeling.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Immunocytology on microwave-fixed cells reveals rapid and agonist-specific changes in subcellular accumulation patterns for cAMP or cGMP.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Recent advances in receptor research.Postgraduate medical journal · 1989Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclic nucleotides play a major role in cell signaling, especially in the nervous system. They act as cytoplasmic messengers in a wide range of physiological responses, but the spatial distribution of their sites of action within cells and tissues is not well-known. In the vertebrate retina, there is a class of well-characterized cGMP binding sites which control the permeability of cation channels in the rod outer segments (ROS), while cAMP is involved in several other systems in the inner retina. Biochemical studies of the cGMP-activated permeability in ROS have not distinguished between the subcellular compartments of disk and plasma membrane. By a new method using fluorescein-conjugated cyclic nucleotides, we have found strong cyclic GMP binding to the plasma membrane of the ROS, both on frozen sections of retina and in freshly isolated, leaky ROS. We also found a high density of cGMP binding sites on structures resembling the inner segment calycal processes. Little specific binding could be detected on the disk membranes or on any other retinal layer. In contrast, fluorescent cAMP did not label ROS, but gave a striking pattern of labeling on several deeper layers of the retina. These results suggest that the ROS plasma membrane has a much higher density of cGMP-controlled cation channels than the disk membranes, and point to other retinal layers where cAMP is likely to shape cellular responses. This method opens up novel morphological approaches to the study of cyclic nucleotide regulation.
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