Evidence map›Paper›PMID 25384788›Full record

ArticleThe Journal of physiology2014

Vitamin C and E supplementation alters protein signalling after a strength training session, but not muscle growth during 10 weeks of training.

G Paulsen, H Hamarsland, K T Cumming, R E Johansen, J J Hulmi, E Børsheim, H Wiig, I Garthe, T Raastad

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed, 1 pooled it
11.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed, 1 synthesis or guideline pooled it, 142 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Trial
  4. Trial
  5. Trial
  6. Trial
  7. Trial
  8. Trial
  9. Trial
  10. Trial
  11. Trial
  12. Trial
  13. Trial
  14. Review
  15. A quantitative redox proteome of the human muscle response to exercise.bioRxiv : the preprint server for biology · 2026
    Article
  16. Review
  17. Review
  18. Review
  19. Eccentric Exercise and Muscle Damage: An Introductory Guide.Journal of functional morphology and kinesiology · 2026
    Review
  20. Article

20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

G PaulsenDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway Norwegian Olympic Federation, Oslo, Norway goran.paulsen@olympiatoppen.no.
H HamarslandDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
K T CummingDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
R E JohansenDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
J J HulmiDepartment of Biology of Physical Activity, Neuromuscular Research Center, University of Jyväskylä, Jyväskylä, Finland.
E BørsheimDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway Arkansas Children's Hospital Research Institute, Departments of Pediatrics and Geriatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
H WiigDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
I GartheNorwegian Olympic Federation, Oslo, Norway.
T RaastadDepartment of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
Norwegian School of Sport Sciences · NONorsk Industri (Norway) · NOUniversity of Arkansas for Medical Sciences · USUniversity of Jyväskylä · FI

Funding

RESOURCE CORE 3: ANALYTICAL COREP30AG028718 · NIA · UNIV OF ARKANSAS FOR MED SCIS · PI WEI, JEANNE Y · 2011 to 2015
$5.1M
NIA NIH HHS P30 AG028718NIA NIH HHS P30-AG028718
6 · The paper itself

Abstract

This study investigated the effects of vitamin C and E supplementation on acute responses and adaptations to strength training. Thirty-two recreationally strength-trained men and women were randomly allocated to receive a vitamin C and E supplement (1000 mg day(-1) and 235 mg day(-1), respectively), or a placebo, for 10 weeks. During this period the participants' training involved heavy-load resistance exercise four times per week. Muscle biopsies from m. vastus lateralis were collected, and 1 repetition maximum (1RM) and maximal isometric voluntary contraction force, body composition (dual-energy X-ray absorptiometry), and muscle cross-sectional area (magnetic resonance imaging) were measured before and after the intervention. Furthermore, the cellular responses to a single exercise session were assessed midway in the training period by measurements of muscle protein fractional synthetic rate and phosphorylation of several hypertrophic signalling proteins. Muscle biopsies were obtained from m. vastus lateralis twice before, and 100 and 150 min after, the exercise session (4 × 8RM, leg press and knee-extension). The supplementation did not affect the increase in muscle mass or the acute change in protein synthesis, but it hampered certain strength increases (biceps curl). Moreover, increased phosphorylation of p38 mitogen-activated protein kinase, Extracellular signal-regulated protein kinases 1 and 2 and p70S6 kinase after the exercise session was blunted by vitamin C and E supplementation. The total ubiquitination levels after the exercise session, however, were lower with vitamin C and E than placebo. We concluded that vitamin C and E supplementation interfered with the acute cellular response to heavy-load resistance exercise and demonstrated tentative long-term negative effects on adaptation to strength training.

Indexed as

MAP Kinase Signaling SystemResistance TrainingAdaptation, PhysiologicalAdultAscorbic AcidDietary SupplementsFemaleHumansIsometric ContractionMaleMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Muscle ProteinsMuscle, Skeletalp38 Mitogen-Activated Protein KinasesRibosomal Protein S6 Kinases, 70-kDaAscorbic AcidMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Muscle Proteinsp38 Mitogen-Activated Protein KinasesRibosomal Protein S6 Kinases, 70-kDaVitamin EVitamins

Identifiers

PMID25384788
PMCPMC4270502
OpenAlexW1598106197

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.