ArticleJournal of bacteriology2015
The EcoKI type I restriction-modification system in Escherichia coli affects but is not an absolute barrier for conjugation.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- Type I restriction-modification systems regulate gene expression through DNA topology.Nucleic acids research · 2026Article
- Eco-evolutionary feedbacks drive the co-occurrence of restriction-modification systems and antimicrobial resistance genes in bacteria.PLoS biology · 2026Article
- Replication-coupled hemimethylation inFrontiers in microbiology · 2026Review
- Biology of host-dependent restriction-modification in prokaryotes.EcoSal Plus · 2025Review
- Development of the Efficient Electroporation Protocol forInternational journal of molecular sciences · 2025Article
- Conjugation-based genetic manipulation ofmBio · 2025Article
- Systematic, high-throughput characterization of bacteriophage gene essentiality on diverse hosts.Cell host & microbe · 2025Article
- The group AInfection and immunity · 2025Article
- Various plasmid strategies limit the effect of bacterial restriction-modification systems against conjugation.Nucleic acids research · 2024Article
- Assessing the Role of Bacterial Innate and Adaptive Immunity as Barriers to Conjugative Plasmids.Molecular biology and evolution · 2024Article
- Type I BREX system defends against antibiotic-resistant plasmids inAntimicrobial agents and chemotherapy · 2024Article
- Molecular Characteristics and Antimicrobial Susceptibility Profiles ofInfection and drug resistance · 2024Article
- Functional prediction of proteins from the human gut archaeome.ISME communications · 2024Article
- Genetic basis of I-complex plasmid stability and conjugation.PLoS genetics · 2023Article
- Delineation of a lactococcal conjugation system reveals a restriction-modification evasion system.Microbial biotechnology · 2023Article
- Conjugation's Toolkit: the Roles of Nonstructural Proteins in Bacterial Sex.Journal of bacteriology · 2023Review
- The DNA Phosphorothioation Restriction-Modification System Influences the Antimicrobial Resistance of Pathogenic Bacteria.Microbiology spectrum · 2023Article
- Plasmid co-infection: linking biological mechanisms to ecological and evolutionary dynamics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Evolutionary Ecology and Interplay of Prokaryotic Innate and Adaptive Immune Systems.Current biology : CB · 2020Review
- ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range.PLoS genetics · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid evolution of bacteria is crucial to their survival and is caused by exchange, transfer, and uptake of DNA, among other things. Conjugation is one of the main mechanisms by which bacteria share their DNA, and it is thought to be controlled by varied bacterial immune systems. Contradictory results about restriction-modification systems based on phenotypic studies have been presented as reasons for a barrier to conjugation with and other means of uptake of exogenous DNA. In this study, we show that inactivation of the R.EcoKI restriction enzyme in strain Escherichia coli K-12 strain MG1655 increases the conjugational transfer of plasmid pOLA52, which carriers two EcoKI recognition sites. Interestingly, the results were not absolute, and uptake of unmethylated pOLA52 was still observed in the wild-type strain (with an intact hsdR gene) but at a reduction of 85% compared to the uptake of the mutant recipient with a disrupted hsdR gene. This leads to the conclusion that EcoKI restriction-modification affects the uptake of DNA by conjugation but is not a major barrier to plasmid transfer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.