Evidence map›Paper›PMID 25384243›Full record

ArticleNature methods2015

Grease matrix as a versatile carrier of proteins for serial crystallography.

Michihiro Sugahara, Eiichi Mizohata, Eriko Nango, Mamoru Suzuki, Tomoyuki Tanaka, Tetsuya Masuda, Rie Tanaka, Tatsuro Shimamura, Yoshiki Tanaka, Chiyo Suno and 14 more

Abstract read
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In one paragraph

Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.

0numbers the graph read from it
0cells of the map it votes in
118citing papers in PubMed
12.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

118 citing papers in PubMed, 223 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026
    Review
  9. Article
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  11. Review
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  13. Review
  14. Article
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  18. Article
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  20. Article

58 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors at 5 institutions in 1 country.

Michihiro SugaharaRIKEN SPring-8 Center, Sayo, Japan.
Eiichi MizohataDepartment of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Japan.
Eriko NangoRIKEN SPring-8 Center, Sayo, Japan.
Mamoru Suzuki1] RIKEN SPring-8 Center, Sayo, Japan. [2] Laboratory of Supramolecular Crystallography, Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, Japan.
Tomoyuki TanakaRIKEN SPring-8 Center, Sayo, Japan.
Tetsuya Masuda1] RIKEN SPring-8 Center, Sayo, Japan. [2] Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Japan.
Rie TanakaRIKEN SPring-8 Center, Sayo, Japan.
Tatsuro ShimamuraDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Yoshiki TanakaDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Chiyo SunoDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Kentaro IharaDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
Dongqing PanDepartment of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Japan.
Keisuke KakinouchiJapan Science and Technology Agency (JST)-Exploratory Research for Advanced Technology (ERATO), Lipid Active Structure Project, Toyonaka, Japan.
Shigeru SugiyamaJapan Science and Technology Agency (JST)-Exploratory Research for Advanced Technology (ERATO), Lipid Active Structure Project, Toyonaka, Japan.
Michio MurataJapan Science and Technology Agency (JST)-Exploratory Research for Advanced Technology (ERATO), Lipid Active Structure Project, Toyonaka, Japan.
Tsuyoshi InoueDepartment of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Japan.
Kensuke TonoJapan Synchrotron Radiation Research Institute, Sayo, Japan.
Changyong SongRIKEN SPring-8 Center, Sayo, Japan.
Jaehyun ParkRIKEN SPring-8 Center, Sayo, Japan.
Takashi KameshimaJapan Synchrotron Radiation Research Institute, Sayo, Japan.
Takaki HatsuiRIKEN SPring-8 Center, Sayo, Japan.
Yasumasa JotiJapan Synchrotron Radiation Research Institute, Sayo, Japan.
Makina YabashiRIKEN SPring-8 Center, Sayo, Japan.ORCID 0000000224721684
So Iwata1] RIKEN SPring-8 Center, Sayo, Japan. [2] Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
SPring-8 · JPKyoto University · JPJapan Science and Technology Agency · JPJapan Synchrotron Radiation Research Institute · JPThe University of Osaka · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

Indexed as

LubricantsAldose-Ketose IsomerasesCrystallizationCrystallography, X-RayFatty Acid Binding Protein 3Fatty Acid-Binding ProteinsLasersMineral OilMuramidasePlant ProteinsProteinsAldose-Ketose IsomerasesFABP3 protein, humanFatty Acid Binding Protein 3Fatty Acid-Binding ProteinsLubricantsMineral OilMuramidasePlant ProteinsProteinsthaumatin protein, plantxylose isomerase

Identifiers

PMID25384243
OpenAlexW2002867805

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.