ArticleBeilstein journal of nanotechnology2014
Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy.
Article in Beilstein journal of nanotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Uptake of gold nanoparticles in HeLa cells observed by confocal microscopy shows dependency on particle size and shape.European biophysics journal : EBJ · 2026Article
- Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures.Journal of functional biomaterials · 2024Review
- Review
- Understanding and improving assays for cytotoxicity of nanoparticles: what really matters?RSC advances · 2018Article
- Engineering of nanoparticle size via electrohydrodynamic jetting.Bioengineering & translational medicine · 2016Article
- Semi-automated quantification of living cells with internalized nanostructures.Journal of nanobiotechnology · 2016Article
- A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes.Beilstein journal of nanotechnology · 2015Article
- Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells.Beilstein journal of nanotechnology · 2014Article
- Synthesis and characterization of fluorescence-labelled silica core-shell and noble metal-decorated ceria nanoparticles.Beilstein journal of nanotechnology · 2014Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Particle_in_Cell-3D is a powerful method to quantify the cellular uptake of nanoparticles. It combines the advantages of confocal fluorescence microscopy with fast and precise semi-automatic image analysis. In this work we present how this method was applied to investigate the impact of 310 nm silica nanoparticles on human vascular endothelial cells (HUVEC) in comparison to a cancer cell line derived from the cervix carcinoma (HeLa). The absolute number of intracellular silica nanoparticles within the first 24 h was determined and shown to be cell type-dependent. As a second case study, Particle_in_Cell-3D was used to assess the uptake kinetics of 8 nm and 30 nm ceria nanoparticles interacting with human microvascular endothelial cells (HMEC-1). These small nanoparticles formed agglomerates in biological medium, and the particles that were in effective contact with cells had a mean diameter of 417 nm and 316 nm, respectively. A significant particle size-dependent effect was observed after 48 h of interaction, and the number of intracellular particles was more than four times larger for the 316 nm agglomerates. Interestingly, our results show that for both particle sizes there is a maximum dose of intracellular nanoparticles at about 24 h. One of the causes for such an interesting and unusual uptake behavior could be cell division.
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