ArticleApplications in plant sciences2014
A molecular identification protocol for roots of boreal forest tree species.
Article in Applications in plant sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Design and application of species-specific primers to Quercus cerris roots' identification in urban forests.Scientific reports · 2025Article
- Digital PCR enables direct root biomass quantification and species profiling in soil samples.Plant physiology · 2025Article
- Expanding and testing fluorescent amplified fragment length polymorphisms for identifying roots of boreal forest plant species.Applications in plant sciences · 2019Article
- Article
- Biases underlying species detection using fluorescent amplified-fragment length polymorphisms yielded from roots.Plant methods · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledPREMISE OF THE STUDY: Roots play a key role in many ecological processes, yet our ability to identify species from bulk root samples is limited. Molecular tools may be used to identify species from root samples, but they have not yet been developed for most systems. Here we present a PCR-based method previously used to identify roots of grassland species, modified for use in boreal forests. •
methodsWe used repeatable interspecific size differences in fluorescent amplified fragment length polymorphisms of three noncoding chloroplast DNA regions to identify seven woody species common to boreal forests in Alberta, Canada. •
resultsAbies balsamea, Alnus crispa, Betula papyrifera, Pinus contorta, and Populus tremuloides were identifiable to species, while Picea glauca and Picea mariana were identifiable to genus. In mixtures of known composition of foliar DNA, species were identified with 98% accuracy using one region. Mixed root samples of unknown composition were identified with 100% accuracy; four species were identified using one region, while three species were identified using two regions. • DISCUSSION: This methodology is accurate, efficient, and inexpensive, and thus a valuable approach for ecological studies of roots. Furthermore, this method has now been validated for both grassland and boreal forest systems, and thus may also have applications in any plant community.
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