Evidence map›Paper›PMID 25383253›Full record

ArticleOptical materials express2014

Enhanced emission of fluorophores on shrink-induced wrinkled composite structures.

Himanshu Sharma, Michelle A Digman, Natasha Felsinger, Enrico Gratton, Michelle Khine

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Optical materials express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 16 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Himanshu SharmaDeparment of Chemical Engineering & Materials Science, University of California, Irvine, California 92697, USA.
Michelle A DigmanDepartment of Developmental and Cell Biology, Laboratory for Fluorescence Dynamics, University of California, Irvine, California 92697, USA ; Department of Biomedical Engineering, Laboratory for Fluorescence Dynamics, University of California, Irvine, California 92697, USA.
Natasha FelsingerDepartment of Biomedical Engineering, University of California, Irvine, California 92697, USA.
Enrico GrattonDepartment of Biomedical Engineering, Laboratory for Fluorescence Dynamics, University of California, Irvine, California 92697, USA.
Michelle KhineDeparment of Chemical Engineering & Materials Science, University of California, Irvine, California 92697, USA ; Department of Biomedical Engineering, University of California, Irvine, California 92697, USA.
University of California, Irvine · US

Funding

Uncover Strategies Used By Biological SystemsP50GM076516 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI LANDER, ARTHUR D · 2007 to 2016
$25.1M
YEAST RIBOSOMAL STALK COMPOSITION IN VIVO BY TWO-PHOTON FCS AND PCH ANALYSISP41RR003155 · NCRR · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI GRATTON, ENRICO · 1986 to 2011
$14.9M
TRD4: Phasor analysisP41GM103540 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GRATTON, ENRICO · 2012 to 2020
$12.5M
Shrink Induced Manufacturing Platform for Low-Cost Diagnostics (SIMPL-CD)DP2OD007283 · OD · UNIVERSITY OF CALIFORNIA-IRVINE · PI KHINE, MICHELLE · 2010 to 2013
$2.4M
NCRR NIH HHS P41 RR003155NIGMS NIH HHS P41 GM103540NIGMS NIH HHS P50 GM076516NIH HHS DP2 OD007283
6 · The paper itself

Abstract

We introduce a manufacturable and scalable method for creating tunable wrinkled ferromagnetic-metallic structures to enhance fluorescence signals. Thin layers of nickel (Ni) and gold (Au) were deposited onto a pre-stressed thermoplastic (shrink wrap film) polymer. Heating briefly forced the metal films to buckle when the thermoplastic retracted, resulting in multi-scale composite 'wrinkles'. This is the first demonstration of leveraging the plasmons in such hybrid nanostructures by metal enhanced fluorescence (MEF) in the near-infrared wavelengths. We observed more than three orders of magnitude enhancement in the fluorescence signal of a single molecule of goat anti-mouse immunoglobulin G (IgG) antibody conjugated to fluorescein isothiocyanate, FITC, (FITC-IgG) by two-photon excitation with these structures. These large enhancements in the fluorescence signal at the nanoscale gaps between the composite wrinkles corresponded to shortened lifetimes due to localized surface plasmons. To characterize these structures, we combined fluctuation correlation spectroscopy (FCS), fluorescence lifetime imaging microscopy (FLIM), and two-photon microscopy to spatially and temporally map the hot spots with high resolution.

Identifiers

PMID25383253
PMCPMC4220269
OpenAlexW2023855687

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.