ArticleXenotransplantation
Glucose metabolism in pigs expressing human genes under an insulin promoter.
Article in Xenotransplantation. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Contributions of Europeans to Xenotransplantation Research: 2. Pig Islet and Cell Xenotransplantation.Transplant international : official journal of the European Society for Organ Transplantation · 2025Review
- Double transgenic neonatal porcine islets as an alternative source for beta cell replacement therapy.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Pig Xenotransplantation in Beta Cell Replacement: Addressing Challenges and Harnessing Potential for Type 1 Diabetes Therapy.Transplant international : official journal of the European Society for Organ Transplantation · 2024Review
- Cutting edge of immune response and immunosuppressants in allogeneic and xenogeneic islet transplantation.Frontiers in immunology · 2024Review
- A brief review of the current status of pig islet xenotransplantation.Frontiers in immunology · 2024Review
- Characterisation of transgenic pigs expressing a human T cell-depleting anti-CD2 monoclonal antibody.Xenotransplantation · 2023Article
- Current Topics of Relevance to the Xenotransplantation of Free Pig Islets.Frontiers in immunology · 2022Review
- The complex functioning of the complement system in xenotransplantation.Xenotransplantation · 2019Review
- Will Genetic Engineering Carry Xenotransplantation of Pig Islets to the Clinic?Current diabetes reports · 2018Review
- [Research progress on the donor cell sources of pancreatic islet transplantation for treatment of diabetes mellitus].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018Review
- The Future of Islet Transplantation Is Now.Frontiers in medicine · 2018Review
- Pig-to-Primate Islet Xenotransplantation: Past, Present, and Future.Cell transplantation · 2017Review
- Gene Editing, Gene Therapy, and Cell Xenotransplantation: Cell Transplantation Across Species.Current transplantation reports · 2017Review
- Progress in Clinical Encapsulated Islet Xenotransplantation.Transplantation · 2016Review
- The role of genetically engineered pigs in xenotransplantation research.The Journal of pathology · 2016Review
- Use of genetically-engineered pig donors in islet transplantation.World journal of transplantation · 2015Review
- Re-engineering islet cell transplantation.Pharmacological research · 2015Review
- Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.Transplant immunology · 2015Article
- Recent advances in understanding xenotransplantation: implications for the clinic.Expert review of clinical immunology · 2015Article
- Pig-to-monkey islet xenotransplantation using multi-transgenic pigs.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2014Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
backgroundXenotransplantation of porcine islets can reverse diabetes in non-human primates. The remaining hurdles for clinical application include safe and effective T-cell-directed immunosuppression, but protection against the innate immune system and coagulation dysfunction may be more difficult to achieve. Islet-targeted genetic manipulation of islet-source pigs represents a powerful tool to protect against graft loss. However, whether these genetic alterations would impair islet function is unknown.
methodsOn a background of α1,3-galactosyltransferase gene-knockout (GTKO)/human (h)CD46, additional genes (hCD39, human tissue factor pathway inhibitor, porcine CTLA4-Ig) were inserted in different combinations under an insulin promoter to promote expression in islets (confirmed by immunofluorescence). Seven pigs were tested for baseline and glucose/arginine-challenged levels of glucose, insulin, C-peptide, and glucagon.
resultsThis preliminary study did not show definite evidence of β-cell deficiencies, even when three transgenes were expressed under the insulin promoter. Of seven animals, all were normoglycemic at fasting, and five of seven had normal glucose disposal rates after challenge. All animals exhibited insulin, C-peptide, and glucagon responses to both glucose and arginine challenge; however, significant interindividual variation was observed.
conclusionsMultiple islet-targeted transgenic expression was not associated with an overtly detrimental effect on islet function, suggesting that complex genetic constructs designed for islet protection warrants further testing in islet xenotransplantation models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.