ArticleBrain structure & function2016
The ascending median raphe projections are mainly glutamatergic in the mouse forebrain.
Article in Brain structure & function, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- GABAIBRO neuroscience reports · 2025Article
- Deciphering the Functions of Raphe-Hippocampal Serotonergic and Glutamatergic Circuits and Their Deficits in Alzheimer's Disease.International journal of molecular sciences · 2025Review
- Synaptic Targets and Cellular Sources of CB1 Cannabinoid Receptor and Vesicular Glutamate Transporter-3 Expressing Nerve Terminals in Relation to GABAergic Neurons in the Human Cerebral Cortex.The European journal of neuroscience · 2025Article
- Article
- The Role of Vesicular Glutamate Transporter Type 3 in Social Behavior, with a Focus on the Median Raphe Region.eNeuro · 2024Article
- The Dopaminergic Cells in the Median Raphe Region Regulate Social Behavior in Male Mice.International journal of molecular sciences · 2024Article
- Sex-specific modulation of the medial prefrontal cortex by glutamatergic median raphe neurons.Science advances · 2023Article
- The two tales of hippocampal sharp-wave ripple content: The rigid and the plastic.Progress in neurobiology · 2023Review
- Top-down projections of the prefrontal cortex to the ventral tegmental area, laterodorsal tegmental nucleus, and median raphe nucleus.Brain structure & function · 2022Article
- The role of inhibitory circuits in hippocampal memory processing.Nature reviews. Neuroscience · 2022Review
- Median Raphe Nonserotonergic Neurons Modulate Hippocampal Theta Oscillations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- A New Player in the Hippocampus: A Review on VGLUT3+ Neurons and Their Role in the Regulation of Hippocampal Activity and Behaviour.International journal of molecular sciences · 2022Review
- Article
- Reciprocal Lateral Hypothalamic and Raphe GABAergic Projections Promote Wakefulness.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Serotonin 5-HTPsychopharmacology · 2021Article
- Neurochemically and Hodologically Distinct Ascending VGLUT3 versus Serotonin Subsystems Comprise the r2-The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Activity and Coupling to Hippocampal Oscillations of Median Raphe GABAergic Cells in Awake Mice.Frontiers in neural circuits · 2021Article
- Two Functionally Distinct Serotonergic Projections into Hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Separable gain control of ongoing and evoked activity in the visual cortex by serotonergic input.eLife · 2020Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The median raphe region (MRR) is thought to be serotonergic and plays an important role in the regulation of many cognitive functions. In the hippocampus (HIPP), the MRR exerts a fast excitatory control, partially through glutamatergic transmission, on a subpopulation of GABAergic interneurons that are key regulators of local network activity. However, not all receptors of this connection in the HIPP and in synapses established by MRR in other brain areas are known. Using combined anterograde tracing and immunogold methods, we show that the GluN2A subunit of the NMDA receptor is present in the synapses established by MRR not only in the HIPP, but also in the medial septum (MS) and in the medial prefrontal cortex (mPFC) of the mouse. We estimated similar amounts of NMDA receptors in these synapses established by the MRR and in local adjacent excitatory synapses. Using retrograde tracing and confocal laser scanning microscopy, we found that the majority of the projecting cells of the mouse MRR contain the vesicular glutamate transporter type 3 (vGluT3). Furthermore, using double retrograde tracing, we found that single cells of the MRR can innervate the HIPP and mPFC or the MS and mPFC simultaneously, and these double-projecting cells are also predominantly vGluT3-positive. Our results indicate that the majority of the output of the MRR is glutamatergic and acts through NMDA receptor-containing synapses. This suggests that key forebrain areas receive precisely targeted excitatory input from the MRR, which is able to synchronously modify activity in those regions via individual MRR cells with dual projections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.