ArticleThe Plant cell2014
Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Intron retention dominates alternative splicing reprogramming during anaerobic stress in Chlamydomonas reinhardtii.Planta · 2026Article
- Energy metabolism and adaptation to hypoxia in the non-photosynthetic green alga Leontynka.BMC biology · 2026Article
- Genome-Wide Characterization and Expression Analysis ofBiology · 2025Article
- Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration.Nature communications · 2023Article
- The Role of Acetate Kinase in the Human ParasiteParasitologia (Basel, Switzerland) · 2022Article
- Shared features and reciprocal complementation of the Chlamydomonas and Arabidopsis microbiota.Nature communications · 2022Article
- Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation.Scientific reports · 2021Article
- Review
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- A highly efficient sulfadiazine selection system for the generation of transgenic plants and algae.Plant biotechnology journal · 2019Article
- The mitochondrial alternative oxidase fromThe Journal of biological chemistry · 2019Article
- Concerted Up-regulation of Aldehyde/Alcohol Dehydrogenase (ADHE) and Starch inThe Journal of biological chemistry · 2017Article
- Robust Transgene Expression from Bicistronic mRNA in the Green Alga Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2016Article
- An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.The Plant cell · 2016Article
- Identification of the Elusive Pyruvate Reductase of Chlamydomonas reinhardtii Chloroplasts.Plant & cell physiology · 2016Article
- Critical role of Chlamydomonas reinhardtii ferredoxin-5 in maintaining membrane structure and dark metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Relevance of nutrient media composition for hydrogen production in Chlamydomonas.Photosynthesis research · 2015Review
- Characterization of unusual truncated hemoglobins of Chlamydomonas reinhardtii suggests specialized functions.Planta · 2015Article
- Biochemical and Kinetic Characterization of the Eukaryotic Phosphotransacetylase Class IIa Enzyme from Phytophthora ramorum.Eukaryotic cell · 2015Article
- The involvement of hydrogen-producing and ATP-dependent NADPH-consuming pathways in setting the redox poise in the chloroplast of Chlamydomonas reinhardtii in anoxia.The Journal of biological chemistry · 2015Article
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydomonas reinhardtii insertion mutants disrupted for genes encoding acetate kinases (EC 2.7.2.1) (ACK1 and ACK2) and a phosphate acetyltransferase (EC 2.3.1.8) (PAT2, but not PAT1) were isolated to characterize fermentative acetate production. ACK1 and PAT2 were localized to chloroplasts, while ACK2 and PAT1 were shown to be in mitochondria. Characterization of the mutants showed that PAT2 and ACK1 activity in chloroplasts plays a dominant role (relative to ACK2 and PAT1 in mitochondria) in producing acetate under dark, anoxic conditions and, surprisingly, also suggested that Chlamydomonas has other pathways that generate acetate in the absence of ACK activity. We identified a number of proteins associated with alternative pathways for acetate production that are encoded on the Chlamydomonas genome. Furthermore, we observed that only modest alterations in the accumulation of fermentative products occurred in the ack1, ack2, and ack1 ack2 mutants, which contrasts with the substantial metabolite alterations described in strains devoid of other key fermentation enzymes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.