ArticleApplied and environmental microbiology2015
Pyrosequencing of mcrA and archaeal 16S rRNA genes reveals diversity and substrate preferences of methanogen communities in anaerobic digesters.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 122 citations in OpenAlex.
- Comparative pangenome analysis of methanogenic archaea from diverse ecosystems reveals potential targets for methane mitigation in rumen microbiome.Journal of animal science and technology · 2026Article
- Methanotrophs and methane oxidation in cold wetlands: distribution, ecology, and mechanisms.ISME communications · 2026Review
- Spatial Heterogeneity in Methane Biogeochemistry and Prokaryotic Community Structure in Sub-Arctic Waterbodies in Northern Canada.Environmental microbiology · 2025Article
- Environmental Gradients and Conservation Status Determine the Structure and Carbon-Related Metabolic Potential of the Prokaryotic Communities of Mediterranean Inland Saline Shallow Lakes.Ecology and evolution · 2025Article
- A reliable in vitro rumen culture system and workflow for screening anti-methanogenic compounds.PloS one · 2025Article
- Multivariate comparison of taxonomic, chemical and operational data from 80 different full-scale anaerobic digester-related systems.Biotechnology for biofuels and bioproducts · 2024Article
- Revisiting Microbial Diversity in Hypersaline Microbial Mats from Guerrero Negro for a Better Understanding of Methanogenic Archaeal Communities.Microorganisms · 2023Article
- Differences in the Composition of the Rumen Microbiota of Finishing Beef Cattle Divergently Ranked for Residual Methane Emissions.Frontiers in microbiology · 2022Article
- Microbial Community Successional Changes in a Full-Scale Mesophilic Anaerobic Digester from the Start-Up to the Steady-State Conditions.Microorganisms · 2021Article
- Archaeal community dynamics in biogas fermentation at various temperatures assessed by mcrA amplicon sequencing using different primer pairs.World journal of microbiology & biotechnology · 2021Article
- Characterization and significance of extracellular polymeric substances, reactive oxygen species, and extracellular electron transfer in methanogenic biocathode.Scientific reports · 2021Article
- Article
- Genomic and transcriptomic insights into methanogenesis potential of novel methanogens from mangrove sediments.Microbiome · 2020Article
- Sward type alters the relative abundance of members of the rumen microbial ecosystem in dairy cows.Scientific reports · 2020Article
- Hydrolysis and Methanogenesis in UASB-AnMBR Treating Municipal Wastewater Under Psychrophilic Conditions: Importance of Reactor Configuration and Inoculum.Frontiers in bioengineering and biotechnology · 2020Article
- Replacing Barley and Soybean Meal With By-products, in a Pasture Based Diet, Alters Daily Methane Output and the Rumen Microbial CommunityFrontiers in microbiology · 2020Article
- Diversity and community of methanogens in the large intestine of finishing pigs.BMC microbiology · 2019Article
- Acetate promotes microbial reductive debromination of tetrabromobisphenol A during the startup phase of anaerobic wastewater sludge bioreactors.The Science of the total environment · 2019Article
- Targeting Bacteria and Methanogens To Understand the Role of Residual Slurry as an Inoculant in Stored Liquid Dairy Manure.Applied and environmental microbiology · 2018Article
- Reduction in Methane Emissions From Acidified Dairy Slurry Is Related to Inhibition ofFrontiers in microbiology · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanogenic archaea play a key role in biogas-producing anaerobic digestion and yet remain poorly taxonomically characterized. This is in part due to the limitations of low-throughput Sanger sequencing of a single (16S rRNA) gene, which in the past may have undersampled methanogen diversity. In this study, archaeal communities from three sludge digesters in Hong Kong and one wastewater digester in China were examined using high-throughput pyrosequencing of the methyl coenzyme M reductase (mcrA) and 16S rRNA genes. Methanobacteriales, Methanomicrobiales, and Methanosarcinales were detected in each digester, indicating that both hydrogenotrophic and acetoclastic methanogenesis was occurring. Two sludge digesters had similar community structures, likely due to their similar design and feedstock. Taxonomic classification of the mcrA genes suggested that these digesters were dominated by acetoclastic methanogens, particularly Methanosarcinales, while the other digesters were dominated by hydrogenotrophic Methanomicrobiales. The proposed euryarchaeotal order Methanomassiliicoccales and the uncultured WSA2 group were detected with the 16S rRNA gene, and potential mcrA genes for these groups were identified. 16S rRNA gene sequencing also recovered several crenarchaeotal groups potentially involved in the initial anaerobic digestion processes. Overall, the two genes produced different taxonomic profiles for the digesters, while greater methanogen richness was detected using the mcrA gene, supporting the use of this functional gene as a complement to the 16S rRNA gene to better assess methanogen diversity. A significant positive correlation was detected between methane production and the abundance of mcrA transcripts in digesters treating sludge and wastewater samples, supporting the mcrA gene as a biomarker for methane yield.
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