ArticleApplied and environmental microbiology2015
Glutamate decarboxylase-dependent acid resistance in Brucella spp.: distribution and contribution to fitness under extremely acidic conditions.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Role of Aspartate 86 in the Catalytic Mechanism ofBiochemistry · 2026Article
- Brucella microti and Rodent-Borne Brucellosis: A Neglected Public Health Threat.Zoonoses and public health · 2025Review
- Strain-specific strategies underlie convergent phosphate solubilization inISME communications · 2025Article
- Diversity and distribution of amino acid decarboxylase enzymes in the human gut bacteria-a bioinformatics investigation.Frontiers in microbiology · 2025Article
- First isolation and identification ofFrontiers in microbiology · 2025Article
- Synthesis and Biological Activity of Homohypotaurine Obtained by the Enzyme-Based Conversion of Homocysteine Sulfinic Acid Using RecombinantMolecules (Basel, Switzerland) · 2024Article
- Genome-wide analysis of Brucella melitensis growth in spleen of infected mice allows rational selection of new vaccine candidates.PLoS pathogens · 2024Article
- Molecular evolution and population genetics of glutamate decarboxylase acid resistance pathway in lactic acid bacteria.Frontiers in genetics · 2023Article
- Brucella ceti and Brucella pinnipedialis genome characterization unveils genetic features that highlight their zoonotic potential.MicrobiologyOpen · 2022Article
- The Retrospective on AtypicalMicroorganisms · 2022Review
- Genome-guided prediction of acid resistance mechanisms in acidophilic methanotrophs of phylogenetically deep-rooted Verrucomicrobia isolated from geothermal environments.Frontiers in microbiology · 2022Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Comparative Genome-Wide Transcriptome Analysis ofFrontiers in microbiology · 2021Article
- Molecular Analysis of Glutamate Decarboxylases inFrontiers in microbiology · 2021Article
- Enzymatic kinetic resolution of desmethylphosphinothricin indicates that phosphinic group is a bioisostere of carboxyl group.Communications chemistry · 2020Article
- Characterizing the transport and utilization of the neurotransmitter GABA in the bacterial pathogen Brucella abortus.PloS one · 2020Article
- Lethality ofVirulence · 2019Article
- Immune Response to MucosalFrontiers in immunology · 2019Review
- PknG supports mycobacterial adaptation in acidic environment.Molecular and cellular biochemistry · 2018Article
- Brucellosis: Evolution and expected comeback.International journal of veterinary science and medicine · 2018Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella is an expanding genus of major zoonotic pathogens, including at least 10 genetically very close species occupying a wide range of niches from soil to wildlife, livestock, and humans. Recently, we have shown that in the new species Brucella microti, the glutamate decarboxylase (Gad)-dependent system (GAD system) contributes to survival at a pH of 2.5 and also to infection in mice by the oral route. In order to study the functionality of the GAD system in the genus Brucella, 47 isolates, representative of all known species and strains of this genus, and 16 strains of the closest neighbor genus, Ochrobactrum, were studied using microbiological, biochemical, and genetic approaches. In agreement with the genome sequences, the GAD system of classical species was not functional, unlike that of most strains of Brucella ceti, Brucella pinnipedialis, and newly described species (B. microti, Brucella inopinata BO1, B. inopinata-like BO2, and Brucella sp. isolated from bullfrogs). In the presence of glutamate, these species were more acid resistant in vitro than classical terrestrial brucellae. Expression in trans of the gad locus from representative Brucella species in the Escherichia coli MG1655 mutant strain lacking the GAD system restored the acid-resistant phenotype. The highly conserved GAD system of the newly described or atypical Brucella species may play an important role in their adaptation to acidic external and host environments. Furthermore, the GAD phenotype was shown to be a useful diagnostic tool to distinguish these latter Brucella strains from Ochrobactrum and from classical terrestrial pathogenic Brucella species, which are GAD negative.
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