ArticleMolecular biotechnology2015
Efficient differentiation of human embryonic stem cells toward dopaminergic neurons using recombinant LMX1A factor.
Article in Molecular biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
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10 citing papers in PubMed, 20 citations in OpenAlex.
- Cell-type-specific effects of genetic variation on chromatin accessibility during human neuronal differentiation.Nature neuroscience · 2021Article
- An Easy and Fast Method for Production of Chinese Hamster Ovary Cell Line Expressing and Secreting Human Recombinant Activin A.Cell journal · 2020Article
- Gene Expression Patterns of Royan Human Embryonic Stem Cells Correlate with Their Propensity and Culture Systems.Cell journal · 2019Article
- Discovery of Novel Cell Surface Markers for Purification of Embryonic Dopamine Progenitors for Transplantation in Parkinson's Disease Animal Models.Molecular & cellular proteomics : MCP · 2018Article
- Common pitfalls of stem cell differentiation: a guide to improving protocols for neurodegenerative disease models and research.Cellular and molecular life sciences : CMLS · 2016Review
- Whole-transcriptome analysis of chordoma of the skull base.Virchows Archiv : an international journal of pathology · 2016Article
- In Vitro Models for Neurogenesis.Cold Spring Harbor perspectives in biology · 2016Review
- Article
- Neural Progenitor Cells Derived from Human Embryonic Stem Cells as an Origin of Dopaminergic Neurons.Stem cells international · 2015Article
- Effect of sevoflurane anesthesia on the comprehensive mRNA expression profile of the mouse hippocampus.Medical gas researchArticle
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct differentiation of dopaminergic (DA) neurons from human pluripotent stem cells (hPSCs) in the absence of gene manipulation is the most desired alternative to clinical treatment of Parkinson disease. Protein transduction-based methods could be efficient, safe approaches to enhance direct differentiation of human embryonic stem cells (hESCs) to DA neurons. In the present study, we compared the differentiation efficiency of DA neurons from hESCs with and without the application of LIM homeobox transcription factor 1 alpha (LMX1A), a master regulatory protein in the development of the midbrain neurons and SHH proteins. The results obtained revealed that the treatment of hESCs with recombinant LMX1A (rLMX1A) protein along with dual SMAD inhibition led to higher expression of LMX1B, LMX1A, FOXA2, PITX3, EN1, and WNT1 effector endogenous genes and two-fold expression of PITX3. Moreover, the highest expression level of PITX3 and TH was observed when rLMX1A was added to the induction medium supplemented with SHH. To our best knowledge, this is the first report demonstrating the application of TAT-LMX1A recombinant protein to enhance hESC differentiation to DA as shown by the expression of DA specific makers. These findings pave the way for enhancing the differentiation of hESCs to DA neurons safely and efficiently without genetic modification.
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