ArticlePloS one2014
Decreased microRNA-125a-3p contributes to upregulation of p38 MAPK in rat trigeminal ganglions with orofacial inflammatory pain.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Role of microRNAs in Pulp Inflammation.Cells · 2021Pooled it
- Role of ncRNAs in the Development of Chronic Pain.Non-coding RNA · 2025Review
- Bioinformatic Analysis from a Descriptive Profile of miRNAs in Chronic Migraine.International journal of molecular sciences · 2024Article
- Identifying circRNA-miRNA-mRNA Regulatory Networks in Chemotherapy-Induced Peripheral Neuropathy.Current issues in molecular biology · 2023Article
- The Role of miRNAs in Neuropathic Pain.Biomedicines · 2023Review
- Transcriptome-wide analysis of trigeminal ganglion and subnucleus caudalis in a mouse model of chronic constriction injury-induced trigeminal neuralgia.Frontiers in pharmacology · 2023Article
- Construction and function analysis of the LncRNA-miRNA-mRNA competing endogenous RNA network in autoimmune hepatitis.BMC medical genomics · 2022Article
- MiR-223-3p alleviates trigeminal neuropathic pain in the male mouse by targeting MKNK2 and MAPK/ERK signaling.Brain and behavior · 2022Article
- Epigenetic Connection of the Calcitonin Gene-Related Peptide and Its Potential in Migraine.International journal of molecular sciences · 2022Review
- Interactions Among Non-Coding RNAs and mRNAs in the Trigeminal Ganglion Associated with Neuropathic Pain.Journal of pain research · 2022Article
- Article
- microRNA-Based Network and Pathway Analysis for Neuropathic Pain in Rodent Models.Frontiers in molecular biosciences · 2021Article
- Optimizing Perioperative Use of Opioids: A Multimodal Approach.Current anesthesiology reports · 2020Article
- Long non-coding RNA ANRIL knockdown suppresses apoptosis and pro-inflammatory cytokines while enhancing neurite outgrowth via binding microRNA-125a in a cellular model of Alzheimer's disease.Molecular medicine reports · 2020Article
- Role of Micro-RNA for Pain After Surgery: Narrative Review of Animal and Human Studies.Anesthesia and analgesia · 2020Review
- MicroRNA-125a-3p participates in odontoblastic differentiation of dental pulp stem cells by targeting Fyn.Cytotechnology · 2020Article
- Review
- Melanocortin type 4 receptor-mediated inhibition of A-type KThe Journal of biological chemistry · 2019Article
- NLRP3 inflammasome signaling as an early molecular response is negatively controlled by miR-186 in CFA-induced prosopalgia mice.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2018Article
- Protective Effects of Sinomenine on CFA-Induced Inflammatory Pain in Rats.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Orofacial inflammatory pain is a difficult clinical problem, and the specific molecular mechanisms for this pain remain largely unexplained. The present study aimed to determine the differential expression of microRNAs (miRNAs) and disclose the underlying role of miR-125a-3p in orofacial inflammatory pain induced by complete Freund's adjuvant (CFA). Thirty-two differentially expressed miRNAs were first screened using a microarray chip in ipsilateral trigeminal ganglions (TGs) following CFA injection into the orofacial skin innervated by trigeminal nerve, and a portion of them, including miR-23a*, -24-2*, -26a, -92a, -125a-3p, -183 and -299 were subsequently selected and validated by qPCR. The target genes were predicted based on the miRWalk website and were further analyzed by gene ontology (GO). Further studies revealed miR-125a-3p expression was down-regulated, whereas both the expression of p38 MAPK (mitogen-activated protein kinase) alpha and CGRP (calcitonin gene-related peptide) were up-regulated in ipsilateral TGs at different time points after CFA injection compared with control. Furthermore, mechanistic study revealed that miR-125a-3p negatively regulates p38 alpha gene expression and is positively correlated with the head withdrawal threshold reflecting pain. Luciferase assay showed that binding of miR-125a-3p to the 3'UTR of p38 alpha gene suppressed the transcriptional activity, and overexpression of miR-125a-3p significantly inhibited the p38 alpha mRNA level in ND8/34 cells. Taken together, our results show that miR-125a-3p is negatively correlated with the development and maintenance of orofacial inflammatory pain via regulating p38 MAPK.
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