ArticlePloS one2014
Sperm flagellum volume determines freezability in red deer spermatozoa.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
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- Antioxidant Activity ofVeterinary sciences · 2025Article
- Extender development for optimal cryopreservation of buck sperm to increase reproductive efficiency of goats.Frontiers in veterinary science · 2025Review
- Changes in the Morphology and Antioxidant Status of European Red Deer Sperm Stored in the Epididymides and in a Liquid State.Animals : an open access journal from MDPI · 2024Article
- Editorial: Novel approaches to predict and improve sperm function during semen storage.Frontiers in veterinary science · 2024Article
- Horn size is linked to Sertoli cell efficiency and sperm size homogeneity during sexual development in common eland (Frontiers in cell and developmental biology · 2024Article
- The Quality and Fertilizing Potential of Red Deer (International journal of molecular sciences · 2022Article
- Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS.Animal reproduction · 2022Article
- Comparative Study of Semen Parameters and Hormone Profile in Small-Spotted Catshark (Animals : an open access journal from MDPI · 2021Article
- Plant Extracts as Alternative Additives for Sperm Preservation.Antioxidants (Basel, Switzerland) · 2021Review
- Characterization of Bottlenose DolphinBiology · 2021Article
- Article
- High-energy diet enhances spermatogenic function and increases sperm midpiece length in fallow deer (Royal Society open science · 2019Article
- Article
- Current status and potential of morphometric sperm analysis.Asian journal of andrologyReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The factors affecting the inter-individual differences in sperm freezability is a major line of research in spermatology. Poor sperm freezability is mainly characterised by a low sperm velocity, which in turn is associated with low fertility rates in most animal species. Studies concerning the implications of sperm morphometry on freezability are quite limited, and most of them are based on sperm head size regardless of the structural parts of the flagellum, which provides sperm motility. Here, for the first time, we determined the volumes of the flagellum structures in fresh epididymal red deer spermatozoa using a stereological method under phase contrast microscopy. Sperm samples from thirty-three stags were frozen and classified as good freezers (GF) or bad freezers (BF) at two hours post-thawing using three sperm kinetic parameters which are strongly correlated with fertility in this species. Fourteen stags were clearly identified as GF, whereas nineteen were BF. No significant difference in sperm head size between the two groups was found. On the contrary, the GF exhibited a lower principal piece volume than the BF (6.13 µm3 vs 6.61 µm3, respectively, p = 0.006). The volume of the flagellum structures showed a strong negative relationship with post-thawing sperm velocity. For instance, the volume of the sperm principal piece was negatively correlated with sperm velocity at two hours post-thawing (r = -0.60; p<0.001). Our results clearly show that a higher volume of the sperm principal piece results in poor freezability, and highlights the key role of flagellum size in sperm cryopreservation success.
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